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Glutathione-S-Transferase (GST Tag) antibody

Antigen

Glutathione-S-Transferase (GST Tag)

Clonality Polyclonal
Host
Alternatives

Rabbit

Application
Alternatives Immunohistochemistry (IHC), Western Blotting (WB)
3 references available
Catalog no. ABIN350351
Quantity 100 µl  (Variants)
Price 413.33 $   Plus shipping costs $35.00
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Availability Ships within 7 to 10 Business Days

Additional Information

Swiss-Prot P08515
Immunogen Synthetic peptide from Glutathione-S-transferase (GST) of Schistosoma japonicum conjugated to an immunogenic carrier protein was used as the immunogen.
Format Lyophilized
Description Widely used a s a fusion partner with proteins of interest. Also known as: Glutathione S-transferase class-mu 26 kDa isozyme, GST, Sj26 antigen, SjGST.
Specificity Appears to be specific for GST.

Application Details

Application Notes IHC, WB. A dilution of 1 : 300 to 1 : 2000 is recommended. The optimal dilution should be determined by the end user. Not yet tested in other applications.
Purity whole serum
Storage Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.
Restrictions For Research Use only

Publications

Publications Smith, Davern, Board et al.: "Mr 26,000 antigen of Schistosoma japonicum recognized by resistant WEHI 129/J mice is a parasite glutathione S-transferase." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 83, Issue 22, pp. 8703-7, 1986 (PubMed).

Lim, Ho, Keeling et al.: "Three-dimensional structure of Schistosoma japonicum glutathione S-transferase fused with a six-amino acid conserved neutralizing epitope of gp41 from HIV." in: Protein science : a publication of the Protein Society, Vol. 3, Issue 12, pp. 2233-44, 1995 (PubMed).

McTigue, Williams, Tainer: "Crystal structures of a schistosomal drug and vaccine target: glutathione S-transferase from Schistosoma japonica and its complex with the leading antischistosomal drug praziquantel." in: Journal of molecular biology, Vol. 246, Issue 1, pp. 21-7, 1995 (PubMed).

Alternatives

Alternatives for antigen "Glutathione-S-Transferase (GST Tag)", type "Antibodies"
Hosts Sheep (2), Rabbit (1)
Applications Immunohistochemistry (IHC) (3), Western Blotting (WB) (3)