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Guanine Nucleotide Binding Protein, alpha Transducing 3 (GNAT3) (Internal Region) antibody

Details for Product No. ABIN350355
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Antigen
Synonyms GDCA, Ggust, Gtn, gnat-3
Epitope
Internal Region
(10), (8), (1)
Reactivity
Mouse (Murine), Rat (Rattus)
(25), (14), (14), (14), (12), (12)
Host
Rabbit
(20), (6)
Clonality
Polyclonal
Conjugate
Un-conjugated
(2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application
Immunohistochemistry (IHC), Western Blotting (WB)
(13), (12), (10), (8), (8), (7)
Pubmed 5 references available
Quantity 100 µL
Options
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Catalog No. ABIN350355
454.67 $
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Immunogen A synthetic peptide from the internal region of mouse Gustducin alpha-3 chain (Gnat3, Guanine nucleotide-binding protein G(t) subunit alpha-3) conjugated to an immunogenic carrier protein was used as the antigen. The antigen shares 87% identity with the mouse sequence.
Specificity Specific for GNAT3.
Purification Whole serum
Alternative Name GNAT3
Background Function: Guanine nucleotide-binding protein (G protein) alpha subunit playing a prominent role in bitter and sweet taste transduction as well as in umami (monosodium glutamate, monopotassium glutamate, and inosine monophosphate) taste transduction. Transduction by this alpha subunit involves coupling of specific cell-surface receptors with a cGMP-phosphodiesterase, Activation of phosphodiesterase lowers intracellular levels of cAMP and cGMP which may open a cyclic nucleotide-suppressible cation channel leading to influx of calcium, ultimately leading to release of neurotransmitter. Indeed, denatonuim and strychnine induce transient reduction in cAMP and cGMP in taste tissue, whereas this decrease is inhibited by GNAT3 antibody. Gustducin heterotrimer transduces response to bitter and sweet compounds via regulation of phosphodiesterase for alpha subunit, as well as via activation of phospholipase C for beta and gamma subunits, with ultimate increase inositol trisphosphate and increase of intracellular Calcium. GNAT3 can functionally couple to taste receptors to transmit intracellular signal: receptor heterodimer TAS1R2/TAS1R3 senses sweetness and TAS1R1/TAS1R3 transduces umami taste, whereas the T2R family GPCRs act as bitter sensors. Functions also as lumenal sugar sensors in the gut to control the expression of the Na+-glucose transporter SGLT1 in response to dietaty sugar, as well as the secretion of Glucagon-like peptide-1, GLP-1 and glucose-dependent insulinotropic polypeptide, GIP. Thus, may modulate the gut capacity to absorb sugars, with implications in malabsorption syndromes and diet-related disorders including diabetes and obesity.
Subcellular location: cytoplasm
Tissue specificity: Expressed in taste buds (sensory organs of clustered epithelial cells) of the circumvallate and fungiform papillae of the tongue as well as in palatal taste buds at protein level. Expressed in enteroendocrine cells of the gut, such as in subsets of enteroendocrine cells in the midjejunum and brush cells. Detected also in spermatozoa. Also known as: Gnat3, Guanine nucleotide-binding protein G(t) subunit alpha-3.
Research Area Neurology
Application Notes A dilution of 1 : 300 to 1 : 2000 is recommended.
The optimal dilution should be determined by the end user.
Not yet tested in other applications.
Restrictions For Research Use only
Format Lyophilized
Reconstitution Reconstitute in 500 µL of sterile water. Centrifuge to remove any insoluble material.
Handling Advice Avoid freeze and thaw cycles.
Storage 4 °C/-20 °C
Storage Comment Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.
Expiry Date 12 months
General Wong, Gannon, Margolskee: "Transduction of bitter and sweet taste by gustducin." in: Nature, Vol. 381, Issue 6585, pp. 796-800, 1996 (PubMed).

He, Yasumatsu, Varadarajan et al.: "Umami taste responses are mediated by alpha-transducin and alpha-gustducin." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 24, Issue 35, pp. 7674-80, 2004 (PubMed).

Carninci, Kasukawa, Katayama et al.: "The transcriptional landscape of the mammalian genome." in: Science (New York, N.Y.), Vol. 309, Issue 5740, pp. 1559-63, 2005 (PubMed).

Sutherland, Young, Cooper et al.: "Phenotypic characterization of taste cells of the mouse small intestine." in: American journal of physiology. Gastrointestinal and liver physiology, Vol. 292, Issue 5, pp. G1420-8, 2007 (PubMed).

Margolskee, Dyer, Kokrashvili et al.: "T1R3 and gustducin in gut sense sugars to regulate expression of Na+-glucose cotransporter 1." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 104, Issue 38, pp. 15075-80, 2007 (PubMed).

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