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HA-Tag (AA 119-127) antibody

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Antigen
Epitope
AA 119-127
37
13
3
2
2
2
2
1
1
Reactivity
Tag
374
16
10
2
2
2
1
1
Host
Sheep
165
156
34
28
20
2
Clonality
Polyclonal
Conjugate
Un-conjugated
38
29
24
6
5
4
4
3
3
3
3
3
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Immunohistochemistry (IHC), Western Blotting (WB)
321
183
112
80
62
41
37
15
11
11
9
7
5
5
4
4
3
2
2
2
1
1
1
1
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Immunogen Synthetic peptide {YPYDVPDYA (119-127)} from Influenza A virus (strain A/Equine/Jillin/1/1989 H3N8) conjugated to an immunogenic carrier protein was used as the antigen.
Sequence YPYDVPDYA
Specificity Specific for HA (YPYDVPDYA).
Purification Whole serum
Alternative Name HA tag
Target Type Tag
Background Epitope tag commonly fused with the protein of interest and expressed as a single chain. Also known as: HA tag, hemagglutinin.
Research Area Tags/Labels
Application Notes A dilution of 1 : 300 to 1 : 2000 is recommended.
The optimal dilution should be determined by the end user.
Not yet tested in other applications.
Restrictions For Research Use only
Format Lyophilized
Reconstitution Reconstitute in 100 µL of sterile water. Centrifuge to remove any insoluble material.
Handling Advice Avoid freeze and thaw cycles.
Storage 4 °C/-20 °C
Storage Comment Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.
Expiry Date 12 months
Background publications Smith, Lapedes, de Jong, Bestebroer, Rimmelzwaan, Osterhaus, Fouchier: "Mapping the antigenic and genetic evolution of influenza virus." in: Science (New York, N.Y.), Vol. 305, Issue 5682, pp. 371-6, 2004 (PubMed).

Fitch, Bush, Bender, Cox: "Long term trends in the evolution of H(3) HA1 human influenza type A." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 94, Issue 15, pp. 7712-8, 1997 (PubMed).