Huntingtin Associated Protein 1 (HAP1) (N-Term) antibody

Antigen: Huntingtin Associated Protein 1 (HAP1)

Synonyms: HLP, HAP2, HIP5, hHLP1

Epitope: N-Term

Clonality: Polyclonal

Host: Rabbit

Application: Immunohistochemistry (IHC), Western Blotting (WB)

Catalog no.: ABIN350357

Additional Information

Alternative name: HAP1 (HAP-1, Huntingtin associated protein 1) (n-terminal)

Swiss-Prot: P54256

Immunogen: A synthetic peptide from the n-terminal of rat HAP1 (HAP-1, Huntingtin associated protein 1) conjugated to an immunogenic carrier protein was used as the immunogen. The immunogen shares 95% identity with mouse sequence.

Format: Lyophilized

Description: FUNCTION: Associates specifically with huntingtin. This binding is enhanced by an expanded polyglutamine repeat. TISSUE SPECIFICITY: In the brain, especially in theolfactory bulb and in the brain stem. No detectable expression in peripheral tissues such as lung, testis, spleen, and small intestine. Also known as: HAP-1, HAP 1, Huntingtin associated protein 1.

Specificity: Appears to be specific for HAP1.

Application Details

Application Notes: IHC, WB. A dilution of 1 : 300 to 1 : 2000 is recommended. The optimal dilution should be determined by the end user. Not yet tested in other applications.

Purity: whole serum

Storage: Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.

Restrictions: For Research Use only

Publications

Publications: Li, Li, Sharp et al.: "A huntingtin-associated protein enriched in brain with implications for pathology." in: Nature, Vol. 378, Issue 6555, pp. 398-402, 1995 (PubMed).

Bertaux, Sharp, Ross et al.: "HAP1-huntingtin interactions do not contribute to the molecular pathology in Huntington's disease transgenic mice." in: FEBS letters, Vol. 426, Issue 2, pp. 229-32, 1998 (PubMed).

Nasir, Duan, Nichol et al.: "Gene structure and map location of the murine homolog of the Huntington-associated protein, Hap1." in: Mammalian genome : official journal of the International Mammalian Genome Society, Vol. 9, Issue 7, pp. 565-70, 1998 (PubMed).