Hyperpolarization Activated Cyclic Nucleotide-Gated Potassium Channel 1 (HCN1) (Cytoplasmic) antibody

Antigen: Hyperpolarization Activated Cyclic Nucleotide-Gated Potassium Channel 1 (HCN1)

Synonyms: BCNG1, HAC-2, BCNG-1, HCN1

Binding Site: Cytoplasmic

Clonality: Polyclonal

Host: Rabbit

Reactivity: Please enquire

Conjugate: Un-conjugated

Application: Immunohistochemistry (IHC), Western Blotting (WB)

Catalog no.: ABIN350358

Additional Information

Alternative name: HCN1

UniProt: O60741

Immunogen: A synthetic peptide from cytoplasmic region of human HCN1 conjugated to an immunogenic carrier protein was used as the immunogen. The antigen is homologous in many species including mouse and rat.

Format: Lyophilized

Description: FUNCTION: Hyperpolarization-activated ion channel exhibiting weak selectivity for potassium over sodium ions. Contributes to the native pacemaker currents in heart (If) and in neurons (Ih). Activated by cAMP, and at 10-100 times higher concentrations, also by cGMP. May mediate responses to sour stimuli. SUBUNIT: The potassium channel is probably composed of a homo- or heterotetrameric complex of pore-forming subunits. Heteromultimer with HCN2. Interacts with KCNE2. SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein. TISSUE SPECIFICITY: Detected in brain, in particular in amygdala and hippocampus, while expression in caudate nucleus, corpus callosum, substantia nigra, subthalamic nucleus and thalamus is very low or not detectable. Detected at very low levels in muscle and pancreas. DOMAIN: The segment S4 is probably the voltage-sensor and is characterized by a series of positively charged amino acids at every third position. Also known as: Brain cyclic nucleotide-gated channel 1, BCNG-1, BCNG1, HAC-2, potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 1.

Specificity: Appears to be specific for HCN1.

Application Details

Application Notes: IHC, WB. A dilution of 1 : 300 to 1 : 2000 is recommended. The optimal dilution should be determined by the end user. Not yet tested in other applications.

Purity: whole serum

Storage: Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.

Restrictions: For Research Use only

Publications

Santoro, Grant, Bartsch et al.: "Interactive cloning with the SH3 domain of N-src identifies a new brain specific ion channel protein, with homology to eag and cyclic nucleotide-gated channels." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 94, Issue 26, pp. 14815-20, 1998 (PubMed).

Santoro, Liu, Yao et al.: "Identification of a gene encoding a hyperpolarization-activated pacemaker channel of brain." in: Cell, Vol. 93, Issue 5, pp. 717-29, 1998 (PubMed).

Ludwig, Zong, Jeglitsch et al.: "A family of hyperpolarization-activated mammalian cation channels." in: Nature, Vol. 393, Issue 6685, pp. 587-91, 1998 (PubMed).

Yu, Wu, Potapova et al.: "MinK-related peptide 1: A beta subunit for the HCN ion channel subunit family enhances expression and speeds activation." in: Circulation research, Vol. 88, Issue 12, pp. E84-7, 2001 (PubMed).

Proenza, Tran, Angoli et al.: "Different roles for the cyclic nucleotide binding domain and amino terminus in assembly and expression of hyperpolarization-activated, cyclic nucleotide-gated channels." in: The Journal of biological chemistry, Vol. 277, Issue 33, pp. 29634-42, 2002 (PubMed).

Xue, Marbuan, Li: "Dominant-negative suppression of HCN1- and HCN2-encoded pacemaker currents by an engineered HCN1 construct: insights into structure-function relationships and multimerization." in: Circulation research, Vol. 90, Issue 12, pp. 1267-73, 2002 (PubMed).

Xue, Li: "An external determinant in the S5-P linker of the pacemaker (HCN) channel identified by sulfhydryl modification." in: The Journal of biological chemistry, Vol. 277, Issue 48, pp. 46233-42, 2002 (PubMed).

Schmutz, Martin, Terry et al.: "The DNA sequence and comparative analysis of human chromosome 5." in: Nature, Vol. 431, Issue 7006, pp. 268-74, 2004 (PubMed).

Carninci, Kasukawa, Katayama et al.: "The transcriptional landscape of the mammalian genome." in: Science (New York, N.Y.), Vol. 309, Issue 5740, pp. 1559-63, 2005 (PubMed).

Munton, Tweedie-Cullen, Livingstone-Zatchej et al.: "Qualitative and quantitative analyses of protein phosphorylation in naive and stimulated mouse synaptosomal preparations." in: Molecular & cellular proteomics : MCP, Vol. 6, Issue 2, pp. 283-93, 2007 (PubMed).