Hepcidin Antimicrobial Peptide (HAMP) (Mature) antibody

Antigen: Hepcidin Antimicrobial Peptide (HAMP)

Synonyms: HAMP, HEPC, Hepcidin, DKFZp470L0111, PLTR, HFE2B, LEAP1, LEAP-1, HEPCIDIN, Hepc, Hamp1, Hepc1, hepcidin, MGC137038

Binding Site: Mature

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Conjugate: Un-conjugated

Application: Immunohistochemistry (IHC), Western Blotting (WB)

Catalog no.: ABIN350364

Additional Information

Alternative name: mature Hepcidin (HAMP)

UniProt: P81172

Immunogen: A synthetic peptide from c-terminal of human Hepcidin (HAMP) conjugated to an immunogenic carrier protein has been used as the immunogen.

Format: Lyophilized

Description: FUNCTION: Seems to act as a signaling molecule involved in the maintenance of iron homeostasis. Seems to be required in conjunction with HFE to regulate both intestinal iron absorption and iron storage in macrophages. Has strong antimicrobial activity against E.coli ML35P N.cinerea and weaker against S.epidermidis, S.aureus and group b streptococcus bacteria. Active against the fungus C.albicans. No activity against P.aeruginosa. SUBCELLULAR LOCATION: Secreted. Tissue specificity: Highest expression in liver and to a lesser extent in heart and brain. Low levels in lung, tonsils, salivary gland, trachea, prostate gland, adrenal gland and thyroid gland. Secreted into the urine. Also known as: Hepcidin, Liver-expressed antimicrobial peptide, LEAP-1, Putative liver tumor regressor, HAMP, HEPC, LEAP1.

Specificity: Appears to be specific for Hepcidin.

Application Details

Application Notes: IHC, WB. Use at a dilution of 1:100 to 1: 2000. The optimal dilution should be determined by the end user. Not yet tested in other applications.

Purity: whole serum

Storage: Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.

Restrictions: For Research Use only

Publications

Krause, Neitz, Maegert et al.: "LEAP-1, a novel highly disulfide-bonded human peptide, exhibits antimicrobial activity." in: FEBS letters, Vol. 480, Issue 2-3, pp. 147-50, 2000 (PubMed).

Park, Valore, Waring et al.: "Hepcidin, a urinary antimicrobial peptide synthesized in the liver." in: The Journal of biological chemistry, Vol. 276, Issue 11, pp. 7806-10, 2001 (PubMed).

Clark, Gurney, Abaya et al.: "The secreted protein discovery initiative (SPDI), a large-scale effort to identify novel human secreted and transmembrane proteins: a bioinformatics assessment." in: Genome research, Vol. 13, Issue 10, pp. 2265-70, 2003 (PubMed).

Grimwood, Gordon, Olsen et al.: "The DNA sequence and biology of human chromosome 19." in: Nature, Vol. 428, Issue 6982, pp. 529-35, 2004 (PubMed).

Delatycki, Allen, Gow et al.: "A homozygous HAMP mutation in a multiply consanguineous family with pseudo-dominant juvenile hemochromatosis." in: Clinical genetics, Vol. 65, Issue 5, pp. 378-83, 2004 (PubMed).