Pro-Hepcidin (AA 25-54) antibody

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  • HAMP
  • hepcidin
  • hepcidin antimicrobial peptide 1
  • hepcidin antimicrobial peptide
  • hamp1
  • HAMP
AA 25-54
Immunohistochemistry (IHC), Western Blotting (WB)
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Immunogen A synthetic peptide from aa region 25-54 of pro region of human Hepcidin conjugated to blue carrier protein has been used as the antigen.
Specificity Specific for Hepcidin.
Purification Whole serum
Background Function: Seems to act as a signaling molecule involved in the maintenance of iron homeostasis. Seems to be required in conjunction with HFE to regulate both intestinal iron absorption and iron storage in macrophages. Has strong antimicrobial activity against E.coli ML35P N.cinerea and weaker against S.epidermidis, S.aureus and group b streptococcus bacteria. Active against the fungus C.albicans. No activity against P.aeruginosa.
Subcellular location: Secreted.
Tissue specificity: Highest expression in liver and to a lesser extent in heart and brain. Low levels in lung, tonsils, salivary gland, trachea, prostate gland, adrenal gland and thyroid gland. Secreted into the urine. Also known as: Hepcidin, Liver-expressed antimicrobial peptide, LEAP-1, Putative liver tumor regressor, HAMP, HEPC, LEAP1.
Application Notes Use at a dilution of 1:100 to 1: 2000.
The optimal dilution should be determined by the end user.
Not yet tested in other applications.
Restrictions For Research Use only
Format Lyophilized
Reconstitution Reconstitute in 500 µL of sterile water. Centrifuge to remove any insoluble material.
Handling Advice Avoid freeze and thaw cycles.
Storage 4 °C/-20 °C
Storage Comment Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.
Expiry Date 12 months
Background publications Grimwood, Gordon, Olsen, Terry, Schmutz, Lamerdin, Hellsten, Goodstein, Couronne, Tran-Gyamfi, Aerts, Altherr, Ashworth, Bajorek, Black, Branscomb, Caenepeel, Carrano, Caoile, Chan, Christensen et al.: "The DNA sequence and biology of human chromosome 19. ..." in: Nature, Vol. 428, Issue 6982, pp. 529-35, 2004 (PubMed).

Delatycki, Allen, Gow, MacFarlane, Radomski, Thompson, Hayden, Goldberg, Samuels: "A homozygous HAMP mutation in a multiply consanguineous family with pseudo-dominant juvenile hemochromatosis." in: Clinical genetics, Vol. 65, Issue 5, pp. 378-83, 2004 (PubMed).

Clark, Gurney, Abaya, Baker, Baldwin, Brush, Chen, Chow, Chui, Crowley, Currell, Deuel, Dowd, Eaton, Foster, Grimaldi, Gu, Hass, Heldens, Huang, Kim, Klimowski, Jin, Johnson, Lee, Lewis, Liao, Mark et al.: "The secreted protein discovery initiative (SPDI), a large-scale effort to identify novel human secreted and transmembrane proteins: a bioinformatics assessment. ..." in: Genome research, Vol. 13, Issue 10, pp. 2265-70, 2003 (PubMed).

Park, Valore, Waring, Ganz: "Hepcidin, a urinary antimicrobial peptide synthesized in the liver." in: The Journal of biological chemistry, Vol. 276, Issue 11, pp. 7806-10, 2001 (PubMed).

Krause, Neitz, Maegert, Schulz, Forssmann, Schulz-Knappe, Adermann: "LEAP-1, a novel highly disulfide-bonded human peptide, exhibits antimicrobial activity." in: FEBS letters, Vol. 480, Issue 2-3, pp. 147-50, 2000 (PubMed).

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