His Tag (Tag) antibody

Antigen: His Tag

Synonyms: CaT2, Ecac1

Binding Site: Tag

Clonality: Polyclonal

Host: Sheep

Application: Immunohistochemistry (IHC), Western Blotting (WB)

Catalog no.: ABIN350371

Additional Information

Alternative name: His tag (Histidin, hexa his tag)

UniProt: Q03909

Immunogen: Synthetic hexa His {HHHHHH} conjugated to an immunogenic carrier protein was used as the immunogen.

Format: Lyophilized

Description: A tag commonly fused with the protein of interest and expressed as a single chain. The expressed recombinant protein can then be conveniently purified on Ni-column. Also known as: His tag, Histidin, Hexa His tag, 6x His tag.

Specificity: Appears to be specific for His tag (HHHHHH).

Application Details

Application Notes: IHC, WB. A dilution of 1 : 300 to 1 : 2000 is recommended. The optimal dilution should be determined by the end user. Not yet tested in other applications.

Purity: whole serum

Storage: Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.

Restrictions: For Research Use only

Publications

Fitch, Bush, Bender et al.: "Long term trends in the evolution of H(3) HA1 human influenza type A." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 94, Issue 15, pp. 7712-8, 1997 (PubMed).

Smith, Lapedes, de Jong et al.: "Mapping the antigenic and genetic evolution of influenza virus." in: Science (New York, N.Y.), Vol. 305, Issue 5682, pp. 371-6, 2004 (PubMed).