His Tag antibody

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Antigen
  • AKR1B11
  • AKR1B12
  • ALDRLn
  • ARL-1
  • ARL1
  • HIS
  • HSI
  • aldo-keto reductase family 1, member B10 (aldose reductase)
  • AKR1B10
Reactivity
Tag
616
18
3
1
Host
Sheep
398
178
27
23
4
4
2
1
Clonality
Polyclonal
Conjugate
Un-conjugated
58
44
35
15
13
11
9
8
8
7
7
7
7
7
7
7
6
6
6
5
4
4
4
3
3
3
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
Application
Immunohistochemistry (IHC), Western Blotting (WB)
528
372
160
106
77
74
66
26
12
11
9
7
7
6
6
5
4
4
4
3
2
1
1
1
1
1
1
1
1
1
1
Options
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Immunogen Synthetic hexa His {HHHHHH} conjugated to an immunogenic carrier protein was used as the antigen.
Sequence HHHHHH
Specificity Specific for His tag (HHHHHH).
Purification Whole serum
Target Type Tag
Background A tag commonly fused with the protein of interest and expressed as a single chain. The expressed recombinant protein can then be conveniently purified on Ni-column. Also known as: His tag, Histidin, Hexa His tag, 6x His tag.
Application Notes A dilution of 1 : 300 to 1 : 2000 is recommended.
The optimal dilution should be determined by the end user.
Not yet tested in other applications.
Restrictions For Research Use only
Format Lyophilized
Reconstitution Reconstitute in 100 µL of sterile water. Centrifuge to remove any insoluble material.
Handling Advice Avoid freeze and thaw cycles.
Storage 4 °C/-20 °C
Storage Comment Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.
Expiry Date 12 months
Background publications Smith, Lapedes, de Jong, Bestebroer, Rimmelzwaan, Osterhaus, Fouchier: "Mapping the antigenic and genetic evolution of influenza virus." in: Science (New York, N.Y.), Vol. 305, Issue 5682, pp. 371-6, 2004 (PubMed).

Fitch, Bush, Bender, Cox: "Long term trends in the evolution of H(3) HA1 human influenza type A." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 94, Issue 15, pp. 7712-8, 1997 (PubMed).