You are viewing an incomplete version of our website. Please click to reload the website as full version.

His Tag antibody

Details for Product No. ABIN350371, Supplier: Login to see
Antigen
  • AKR1B11
  • AKR1B12
  • ALDRLn
  • ARL-1
  • ARL1
  • HIS
  • HSI
Reactivity
Tag
479
22
7
3
Host
Sheep
271
180
31
26
2
Clonality
Polyclonal
Conjugate
Un-conjugated
50
38
35
15
12
8
4
4
4
4
4
4
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
Application
Immunohistochemistry (IHC), Western Blotting (WB)
430
274
166
75
62
32
32
13
12
11
9
7
5
4
4
4
3
2
2
2
2
1
1
1
1
1
1
Supplier
Login to see
Supplier Product No.
Login to see
Request

Showcase your results, aid the scientific community, and receive a full refund.

Contribute a validation

Learn more

Immunogen Synthetic hexa His {HHHHHH} conjugated to an immunogenic carrier protein was used as the antigen.
Sequence HHHHHH
Specificity Specific for His tag (HHHHHH).
Purification Whole serum
Target Type Tag
Background A tag commonly fused with the protein of interest and expressed as a single chain. The expressed recombinant protein can then be conveniently purified on Ni-column. Also known as: His tag, Histidin, Hexa His tag, 6x His tag.
Pathways
Application Notes A dilution of 1 : 300 to 1 : 2000 is recommended.
The optimal dilution should be determined by the end user.
Not yet tested in other applications.
Restrictions For Research Use only
Format Lyophilized
Reconstitution Reconstitute in 100 µL of sterile water. Centrifuge to remove any insoluble material.
Handling Advice Avoid freeze and thaw cycles.
Storage 4 °C/-20 °C
Storage Comment Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.
Expiry Date 12 months
Background publications Smith, Lapedes, de Jong et al.: "Mapping the antigenic and genetic evolution of influenza virus." in: Science (New York, N.Y.), Vol. 305, Issue 5682, pp. 371-6, 2004 (PubMed).

Fitch, Bush, Bender et al.: "Long term trends in the evolution of H(3) HA1 human influenza type A." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 94, Issue 15, pp. 7712-8, 1997 (PubMed).