Huntingtin (HTT) (internal) antibody

Antigen: Huntingtin (HTT)

Synonyms: HD, IT15, Hd, Hdh, AI256365, C430023I11Rik, hd, ZHD, HTT, HsapHD, dhtt, DmelCG9995, CG9995, Htt, HDH, SLC6A4, it15, MGC79590, htt, LOC100216476

Epitope: internal

Clonality: Polyclonal

Host: Rabbit

Application: Immunohistochemistry (IHC), Western Blotting (WB)

Catalog no.: ABIN350376

Additional Information

Alternative name: Huntingtin (HTT) (internal region)

Swiss-Prot: P51111

Immunogen: A synthetic peptide from internal part of rat Huntingtin (Huntington disease protein homolog, Htt, HD protein homolog) conjugated to an immunogenic carrier protein was used as the immunogen. The antigen shares 87% identity with mouse and human sequences.

Format: Lyophilized

Description: FUNCTION: May play a role in microtubule-mediated transport or vesicle function. SUBCELLULAR LOCATION: Cytoplasm. Nucleus. TISSUE SPECIFICITY: Expressed to a high degree in all the regions of the brain of adults and in meiotic cells of the testis. In addition, very low levels are detected in various non-neuronal tissues (heart, muscle, liver, lung and kidney). DEVELOPMENTAL STAGE: Identified at high levels in neuronal tissues of embryos as early as day 14.5. This expression remains constant in all further development stages (up to the adult). On the other hand the expression in non-neuronal tissues is down-regulated from stage 17.5 day old embryos. Also known as: Huntington disease protein homolog, Htt, HD protein homolog.

Specificity: Appears to be specific for Huntingtin.

Application Details

Application Notes: IHC, WB. A dilution of 1 : 300 to 1 : 2000 is recommended. The optimal dilution should be determined by the end user. Not yet tested in other applications.

Purity: whole serum

Storage: Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.

Restrictions: For Research Use only

Images

IF on 1 day- old mouse cortical cell culture using Rabbit antibody to internal part of Huntingtin (Huntington disease protein homolog, Htt, HD protein homolog): whole serumat 1: 500 dilution. DAPI counter stained.

IF on 1 day- old mouse cortical cell culture using Rabbit antibody to internal part of Huntingtin (Huntington disease protein homolog, Htt, HD protein homolog): whole serumat 1: 500 dilution. DAPI counter stained appearing in blue.

IF on 1 day- old mouse cortical cell culture using Rabbit antibody to internal part of Huntingtin (Huntington disease protein homolog, Htt, HD protein homolog): whole serumat 1: 500 dilution. DAPI counter stained appearing in blue.

Publications

Publications: Strong, Tagle, Valdes et al.: "Widespread expression of the human and rat Huntington's disease gene in brain and nonneural tissues." in: Nature genetics, Vol. 5, Issue 3, pp. 259-65, 1994 (PubMed).

Schmitt, Baechner, Megow et al.: "Expression of the Huntington disease gene in rodents: cloning the rat homologue and evidence for downregulation in non-neuronal tissues during development." in: Human molecular genetics, Vol. 4, Issue 7, pp. 1173-82, 1996 (PubMed).