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Details for Product No. ABIN350382

Potassium Inwardly-Rectifying Channel, Subfamily J, Member 1 (KCNJ1) (Cytoplasmic Domain) antibody

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Antigen
Synonyms ROMK, ROMK1, KIR1.1, Kcnj, Romk2, Kir1.1, kir1.1, romk1, kcnj1, wu:fl37c05, zgc:63534, MGC68935
Epitope
»Alternatives Cytoplasmic Domain
Reactivity
»Alternatives Human
Host
»Alternatives Rabbit
Clonality Polyclonal
Conjugate
»Alternatives Un-conjugated
Application
»Alternatives Immunohistochemistry (IHC), Western Blotting (WB)
Pubmed 6 references available
Catalog no. ABIN350382
Quantity 100 µL
Price
454.67 $   Plus shipping costs $45.00
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Availability Will be delivered in 7 to 8 Business Days
Immunogen A synthetic peptide from the cytoplasmic domain of human KCNJ1 (ROMK1, Kir1.1) conjugated to an immunogenic carrier protein was used as the antigen. The antigen is shares 92% identity with rat and mouse sequences.
Specificity Specific for Draxin.
Purity whole serum
Alternative Name KCNJ1 (ROMK1, Kir1.1)
Background Function: In the kidney, probably plays a major role in potassium homeostasis. Inward rectifier potassium channels are characterized by a greater tendency to allow potassium to flow into the cell rather than out of it. Their voltage dependence is regulated by the concentration of extracellular potassium, as external potassium is raised, the voltage range of the channel opening shifts to more positive voltages. The inward rectification is mainly due to the blockage of outward current by internal magnesium. This channel is activated by internal ATP and can be blocked by external barium.
Tissue specificity: In the kidney and pancreatic islets. Lower levels in skeletal muscle, pancreas, spleen, brain, heart and liver.
Subcellular location: Membrane, Multi-pass membrane protein. Involvement in disease: Defects in KCNJ1 are the cause of Bartter syndrome type 2 (BS2) also termed hyperprostanglandin E syndrome 2. BS refers to a group of autosomal recessive disorders characterized by impaired salt reabsorption in the thick ascending loop of Henle with pronounced salt wasting, hypokalemic metabolic alkalosis, and varying degrees of hypercalciuria. BS2 is a life-threatening condition beginning in utero, with marked fetal polyuria that leads to polyhydramnios and premature delivery. Another hallmark of BS2 is a marked hypercalciuria and, as a secondary consequence, the development of nephrocalcinosis and osteopenia. Also known as: ATP-sensitive inward rectifier potassium channel 1, Potassium channel, inwardly rectifying subfamily J member 1, ATP-regulated potassium channel ROM-K, Kir1.1, ROMK1.
UniProt Q8NBI3
Application Notes A dilution of 1 : 300 to 1 : 2000 is recommended.
The optimal dilution should be determined by the end user.
Not yet tested in other applications.
Restrictions For Research Use only
Format Lyophilized
Reconstitution Reconstitute in 100 µL of sterile water. Centrifuge to remove any insoluble material.
Handling Advice Avoid freeze and thaw cycles.
Storage -20 °C
Storage Comment Maintain the lyophilised/reconstituted antibodies frozen at -20 °C for long term storage and refrigerated at 2-8 °C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability.
Expiry Date 12 months
Krishnan, Desai, Ward et al.: "Isolation and chromosomal localization of a human ATP-regulated potassium channel." in: Human genetics, Vol. 96, Issue 2, pp. 155-60, 1995 (PubMed).

Shuck, Bock, Benjamin et al.: "Cloning and characterization of multiple forms of the human kidney ROM-K potassium channel." in: The Journal of biological chemistry, Vol. 269, Issue 39, pp. 24261-70, 1994 (PubMed).

Yano, Philipson, Kugler et al.: "Alternative splicing of human inwardly rectifying K+ channel ROMK1 mRNA." in: Molecular pharmacology, Vol. 45, Issue 5, pp. 854-60, 1994 (PubMed).

Simon, Karet, Rodriguez-Soriano et al.: "Genetic heterogeneity of Bartter's syndrome revealed by mutations in the K+ channel, ROMK." in: Nature genetics, Vol. 14, Issue 2, pp. 152-6, 1996 (PubMed).

Bock, Shuck, Benjamin et al.: "Nucleotide sequence analysis of the human KCNJ1 potassium channel locus." in: Gene, Vol. 188, Issue 1, pp. 9-16, 1997 (PubMed).

Sjöblom, Jones, Wood et al.: "The consensus coding sequences of human breast and colorectal cancers." in: Science (New York, N.Y.), Vol. 314, Issue 5797, pp. 268-74, 2006 (PubMed).

Alternatives for antigen "Potassium Inwardly-Rectifying Channel, Subfamily J, Member 1 (KCNJ1)", type "Antibodies"
Hosts (33), (5), (1), (1)
Reactivities (39), (25), (25), (13), (1), (1)
Applications (21), (19), (10), (8), (6), (4), (2), (1), (1)
Conjugates (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Epitopes (16), (4), (4), (2), (2), (1), (1), (1), (1), (1)
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