Potassium Inwardly-Rectifying Channel, Subfamily J, Member 10 (KCNJ10) (C-Term) antibody

Antigen: Potassium Inwardly-Rectifying Channel, Subfamily J, Member 10 (KCNJ10)

Synonyms: KIR1.2, KIR4.1, SESAME, BIRK-10, KCNJ13-PEN, BIR10, BIRK-1, Kir1.2, Kir4.1, kir1.2, kir4.1, birk-10, KCNJ10, MGC152173, MGC145926

Epitope: C-Term

Clonality: Polyclonal

Host: Rabbit

Application: Immunohistochemistry (IHC), Western Blotting (WB)

Catalog no.: ABIN350384

Additional Information

Alternative name: KCNJ10 (Kir1.2) (c-terminal region)

Swiss-Prot: P78508

Immunogen: A synthetic peptide from the c-terminal region of human KCNJ10 (Kir1.2) conjugated to an immunogenic carrier protein was used as the immunogen. The antigen is homologous in mouse and rat.

Format: Lyophilized

Description: FUNCTION: May be responsible for potassium buffering action of glial cells in the brain. Inward rectifier potassium channels are characterized by a greater tendency to allow potassium to flow into the cell rather than out of it. Their voltage dependence is regulated by the concentration of extracellular potassium; as external potassium is raised, the voltage range of the channel opening shifts to more positive voltages. The inward rectification is mainly due to the blockage of outward current by internal magnesium. Can be blocked by extracellular barium and cesium. Subcellular location: Membrane, Multi-pass membrane protein. Also known as: ATP-sensitive inward rectifier potassium channel 10, Potassium channel, inwardly rectifying subfamily J member 10, Inward rectifier K(+) channel Kir1.2, ATP-dependent inwardly rectifying potassium channel Kir4.1.

Specificity: Appears to be specific for KCNJ10.

Application Details

Application Notes: IHC, WB. A dilution of 1 : 300 to 1 : 2000 is recommended. The optimal dilution should be determined by the end user. Not yet tested in other applications.

Purity: whole serum

Storage: Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.

Restrictions: For Research Use only

Publications

Publications: Shuck, Piser, Bock et al.: "Cloning and characterization of two K+ inward rectifier (Kir) 1.1 potassium channel homologs from human kidney (Kir1.2 and Kir1.3)." in: The Journal of biological chemistry, Vol. 272, Issue 1, pp. 586-93, 1997 (PubMed).

Kurschner, Mermelstein, Holden et al.: "CIPP, a novel multivalent PDZ domain protein, selectively interacts with Kir4.0 family members, NMDA receptor subunits, neurexins, and neuroligins." in: Molecular and cellular neurosciences, Vol. 11, Issue 3, pp. 161-72, 1998 (PubMed).

Schoots, Wilson, Ethier et al.: "Co-expression of human Kir3 subunits can yield channels with different functional properties." in: Cellular signalling, Vol. 11, Issue 12, pp. 871-83, 2000 (PubMed).

Li, Head, Timpe: "Identification of an inward rectifier potassium channel gene expressed in mouse cortical astrocytes." in: Glia, Vol. 33, Issue 1, pp. 57-71, 2001 (PubMed).

Gregory, Barlow, McLay et al.: "The DNA sequence and biological annotation of human chromosome 1." in: Nature, Vol. 441, Issue 7091, pp. 315-21, 2006 (PubMed).