Potassium Channel, Inwardly Rectifying Subfamily J Member 12 (KCNJ12) (cytoplasmic domain) antibody

Antigen: Potassium Channel, Inwardly Rectifying Subfamily J Member 12 (KCNJ12)

Synonyms: IRK2, Kir2.1, MGC156606, hIRK, hIRK1, KCNJN1, Kir2.2, Kir2.2v, kcnj12x, FLJ14167, hkir2.2x, MB-IRK2, MGC144838, MGC144839

Epitope: cytoplasmic domain

Clonality: Polyclonal

Host: Rabbit

Application: Immunohistochemistry (IHC), Western Blotting (WB)

Catalog no.: ABIN350387

Additional Information

Alternative name: KCNJ12 (KCNJN1, Kir2.2, IRK2) (cytoplasmic domain)

Swiss-Prot: Q14500

Immunogen: A synthetic peptide from the cytoplasmic domain of human KCNJ12 (KCNJN1, Kir2.2, IRK2) conjugated to an immunogenic carrier protein was used as the immunogen.

Format: Lyophilized

Description: FUNCTION: Probably participates in establishing action potential waveform and excitability of neuronal and muscle tissues. Inward rectifier potassium channels are characterized by a greater tendency to allow potassium to flow into the cell rather than out of it. Their voltage dependence is regulated by the concentration of extracellular potassium; as external potassium is raised, the voltage range of the channel opening shifts to more positive voltages. The inward rectification is mainly due to the blockage of outward current by internal magnesium. Can be blocked by extracellular barium and cesium. Tissue specificity: Heart, brain, placenta, lung, skeletal muscle, and kidney. Diffusely distributed throughout the brain. Subcellular location: Membrane, Multi-pass membrane protein. Also known as: ATP-sensitive inward rectifier potassium channel 12, Potassium channel, inwardly rectifying subfamily J member 12, Inward rectifier K(+) channel Kir2.2, Kir2.2v, IRK2, KCNJN1.

Specificity: Appears to be specific for KCNJ12.

Application Details

Application Notes: IHC, WB. A dilution of 1 : 300 to 1 : 2000 is recommended. The optimal dilution should be determined by the end user. Not yet tested in other applications.

Purity: whole serum

Storage: Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.

Restrictions: For Research Use only

Publications

Publications: Wible, De Biasi, Majumder et al.: "Cloning and functional expression of an inwardly rectifying K+ channel from human atrium." in: Circulation research, Vol. 76, Issue 3, pp. 343-50, 1995 (PubMed).

Namba, Inagaki, Gonoi et al.: "Kir2.2v: a possible negative regulator of the inwardly rectifying K+ channel Kir2.2." in: FEBS letters, Vol. 386, Issue 2-3, pp. 211-4, 1996 (PubMed).

Gallagher, Forget: "An alternate promoter directs expression of a truncated, muscle-specific isoform of the human ankyrin 1 gene." in: The Journal of biological chemistry, Vol. 273, Issue 3, pp. 1339-48, 1998 (PubMed).

Kaibara, Ishihara, Doi et al.: "Identification of human Kir2.2 (KCNJ12) gene encoding functional inward rectifier potassium channel in both mammalian cells and Xenopus oocytes." in: FEBS letters, Vol. 531, Issue 2, pp. 250-4, 2002 (PubMed).

Dephoure, Zhou, Villuen et al.: "A quantitative atlas of mitotic phosphorylation." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 105, Issue 31, pp. 10762-7, 2008 (PubMed).