Potassium Channel, Inwardly Rectifying Subfamily J Member 12 (KCNJ12) (cytoplasmic domain) antibody

Antigen: Potassium Channel, Inwardly Rectifying Subfamily J Member 12 (KCNJ12)

Synonyms: IRK2, Kir2.1, MGC156606, hIRK, hIRK1, KCNJN1, Kir2.2, Kir2.2v, kcnj12x, FLJ14167, hkir2.2x, MB-IRK2, MGC144838, MGC144839

Epitope: cytoplasmic domain

Clonality: Polyclonal

Host: Rabbit

Application: Immunohistochemistry (IHC), Western Blotting (WB)

Catalog no.: ABIN350390

Additional Information

Alternative name: KCNJ12 (KCNJN1, Kir2.2, IRK2) (cytoplasmic domain)

Swiss-Prot: P52188

Immunogen: A synthetic peptide from the cytoplasmic domain of rat KCNJ12 (KCNJN1, Kir2.2, IRK2) conjugated to an immunogenic carrier protein was used as the immunogen. The antigen is homologous in mouse.

Format: Lyophilized

Isotype: IgG  (Matching secondary antibodies)

Description: FUNCTION: Probably participates in establishing action potential waveform and excitability of neuronal and muscle tissues. Inward rectifier potassium channels are characterized by a greater tendency to allow potassium to flow into the cell rather than out of it. Their voltage dependence is regulated by the concentration of extracellular potassium; as external potassium is raised, the voltage range of the channel opening shifts to more positive voltages. The inward rectification is mainly due to the blockage of outward current by internal magnesium. Can be blocked by extracellular barium and cesium. Tissue specificity: Highest level in cerebellum. Moderately found in kidney, forebrain and skeletal muscle. Not detected in uterus, liver and pancreas. Subcellular location: Membrane, Multi-pass membrane protein. Also known as: ATP-sensitive inward rectifier potassium channel 12, Potassium channel, inwardly rectifying subfamily J member 12, Inward rectifier K(+) channel Kir2.2, Kir2.2v, IRK2, KCNJN1.

Specificity: Appears to be specific for KCNJ12.

Application Details

Application Notes: IHC, WB. A concentration of 10-50 µg/ml is recommended. The optimal concentration should be determined by the end user. Not yet tested in other applications.

Purity: IgG

Storage: Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.

Restrictions: For Research Use only

Images

Rabbit antibody to cytoplasmic domain of KCNJ12 (KCNJN1, Kir2.2, IRK2): whole serum (ABIN350389) at a concentration of 30 microgram/ml. Blocked with 1% LFDM for 15 minutes at room temperature with shake, primary antibody incubated for 15 minutes at room temperature, washed 3 times with PBST, 5 minutes each. Secondary antibody was also incubated for 15 minutes at room temperature.

Publications

Publications: Koyama, Morishige, Takahashi et al.: "Molecular cloning, functional expression and localization of a novel inward rectifier potassium channel in the rat brain." in: FEBS letters, Vol. 341, Issue 2-3, pp. 303-7, 1994 (PubMed).

Leonoudakis, Conti, Radeke et al.: "A multiprotein trafficking complex composed of SAP97, CASK, Veli, and Mint1 is associated with inward rectifier Kir2 potassium channels." in: The Journal of biological chemistry, Vol. 279, Issue 18, pp. 19051-63, 2004 (PubMed).