Potassium Inwardly-Rectifying Channel, Subfamily J, Member 4 (KCNJ4) (cytoplasmic domain) antibody

Antigen: Potassium Inwardly-Rectifying Channel, Subfamily J, Member 4 (KCNJ4)

Synonyms: HIR, HRK1, IRK3, HIRK2, Kir2.3, MGC142066, MGC142068, Kcnf2, MB-IRK3, Hirk2, KCNJ4, CKIR2.3

Epitope: cytoplasmic domain

Clonality: Polyclonal

Host: Rabbit

Application: Immunohistochemistry (IHC), Western Blotting (WB)

Catalog no.: ABIN350397

Additional Information

Alternative name: KCNJ4 (Kir2.3, HIRK2, HRK1, HIR) (cytoplasmic domain)

Swiss-Prot: P48050

Immunogen: A synthetic peptide from the cytoplasmic domain of human KCNJ4 (Kir2.3, HIRK2, HRK1, HIR) conjugated to an immunogenic carrier protein was used as the immunogen. The antigen shares 91% identity with rat and mouse sequences.

Format: Lyophilized

Description: FUNCTION: This receptor is controlled by G proteins. Inward rectifier potassium channels are characterized by a greater tendency to allow potassium to flow into the cell rather than out of it. Their voltage dependence is regulated by the concentration of extracellular potassium; as external potassium is raised, the voltage range of the channel opening shifts to more positive voltages. The inward rectification is mainly due to the blockage of outward current by internal magnesium. Can be blocked by extracellular barium and cesium. Tissue specificity: Heart, skeletal muscle, and several different brain regions including the hippocampus. Subcellular location: Membrane, Multi-pass membrane protein. Also known as: Inward rectifier potassium channel 4, Potassium channel, inwardly rectifying subfamily J member 4, Inward rectifier K(+) channel Kir2.3, Hippocampal inward rectifier, HIRK2, HRK1, HIR.

Specificity: Appears to be specific for KCNJ4.

Application Details

Application Notes: IHC, WB. A dilution of 1 : 300 to 1 : 2000 is recommended. The optimal dilution should be determined by the end user. Not yet tested in other applications.

Purity: whole serum

Storage: Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.

Restrictions: For Research Use only

Publications

Publications: Puerier, Radeke, Vandenberg: "Primary structure and characterization of a small-conductance inwardly rectifying potassium channel from human hippocampus." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 91, Issue 13, pp. 6240-4, 1994 (PubMed).

Makhina, Kelly, Lopatin et al.: "Cloning and expression of a novel human brain inward rectifier potassium channel." in: The Journal of biological chemistry, Vol. 269, Issue 32, pp. 20468-74, 1994 (PubMed).

Dunham, Shimizu, Roe et al.: "The DNA sequence of human chromosome 22." in: Nature, Vol. 402, Issue 6761, pp. 489-95, 1999 (PubMed).