Potassium Inwardly-Rectifying Channel, Subfamily J, Member 8 (KCNJ8) (Cytoplasmic Domain) antibody

Antigen: Potassium Inwardly-Rectifying Channel, Subfamily J, Member 8 (KCNJ8)

Synonyms: KIR6.1, uKATP-1, sltr, gnite, slmbr, Kir6.1, AI448900, UKATP1, MGC137639, zkir6.1, si:dkey-183c2.2, kir6.1

Binding Site: Cytoplasmic Domain

Clonality: Polyclonal

Host: Rabbit

Reactivity: Please enquire

Application: Immunohistochemistry (IHC), Western Blotting (WB)

Catalog no.: ABIN350401

Additional Information

Alternative name: KCNJ8 (Kir6.1, uKATP-1)

UniProt: Q15842

Immunogen: A synthetic peptide from the cytoplasmic domain of human KCNJ8 (Kir6.1, uKATP-1) conjugated to an immunogenic carrier protein was used as the immunogen. The antigen is homologous in rat and mouse.

Format: Lyophilized

Isotype: IgG

Description: FUNCTION: This potassium channel is controlled by G proteins. Inward rectifier potassium channels are characterized by a greater tendency to allow potassium to flow into the cell rather than out of it. Their voltage dependence is regulated by the concentration of extracellular potassium; as external potassium is raised, the voltage range of the channel opening shifts to more positive voltages. The inward rectification is mainly due to the blockage of outward current by internal magnesium. Can be blocked by external barium. Subcellular location: Membrane, Multi-pass membrane protein. Also known as: ATP-sensitive inward rectifier potassium channel 8, Potassium channel, inwardly rectifying subfamily J member 8, Inwardly rectifier K(+) channel Kir6.1, uKATP-1.

Specificity: Appears to be specific for KCNJ8.

Application Details

Application Notes: IHC, WB. A concentration of 10-50 µg/ml is recommended. The optimal concentration should be determined by the end user. Not yet tested in other applications.

Purity: IgG

Storage: Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.

Restrictions: For Research Use only

Publications

Inagaki, Inazawa, Seino: "cDNA sequence, gene structure, and chromosomal localization of the human ATP-sensitive potassium channel, uKATP-1, gene (KCNJ8)." in: Genomics, Vol. 30, Issue 1, pp. 102-4, 1996 (PubMed).

Olsen, Blagoev, Gnad et al.: "Global, in vivo, and site-specific phosphorylation dynamics in signaling networks." in: Cell, Vol. 127, Issue 3, pp. 635-48, 2006 (PubMed).

Imami, Sugiyama, Kyono et al.: "Automated phosphoproteome analysis for cultured cancer cells by two-dimensional nanoLC-MS using a calcined titania/C18 biphasic column." in: Analytical sciences : the international journal of the Japan Society for Analytical Chemistry, Vol. 24, Issue 1, pp. 161-6, 2008 (PubMed).