Potassium Inwardly-Rectifying Channel, Subfamily J, Member 9 (KCNJ9) (cytoplasmic domain) antibody

Antigen: Potassium Inwardly-Rectifying Channel, Subfamily J, Member 9 (KCNJ9)

Synonyms: GIRK3, KIR3.3

Epitope: cytoplasmic domain

Clonality: Polyclonal

Host: Rabbit

Application: Immunohistochemistry (IHC), Western Blotting (WB)

Catalog no.: ABIN350402

Additional Information

Alternative name: KCNJ9 (Kir3.3, GIRK3) (cytoplasmic domain)

Swiss-Prot: Q92806

Immunogen: A synthetic peptide from the cytoplasmic domain of human KCNJ9 (Kir3.3, GIRK3) conjugated to an immunogenic carrier protein was used as the immunogen. The antigen is homologous in rat and mouse.

Format: Lyophilized

Description: FUNCTION: This receptor is controlled by G proteins. Inward rectifier potassium channels are characterized by a greater tendency to allow potassium to flow into the cell rather than out of it. Their voltage dependence is regulated by the concentration of extracellular potassium; as external potassium is raised, the voltage range of the channel opening shifts to more positive voltages. The inward rectification is mainly due to the blockage of outward current by internal magnesium. Tissue specificity: Expressed mainly in the brain, some expression in the skeletal muscle. Subcellular location: Membrane, Multi-pass membrane protein. Also known as: G protein-activated inward rectifier potassium channel 3, GIRK3, Potassium channel, inwardly rectifying subfamily J member 9, Inwardly rectifier K(+) channel Kir3.3.

Specificity: Appears to be specific for KCNJ9.

Application Details

Application Notes: IHC, WB (confirmed by recombinant protein). A dilution of 1 : 300 to 1 : 2000 is recommended. The optimal dilution should be determined by the end user. Not yet tested in other applications.

Purity: whole serum

Storage: Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.

Restrictions: For Research Use only

Publications

Publications: Schoots, Wilson, Ethier et al.: "Co-expression of human Kir3 subunits can yield channels with different functional properties." in: Cellular signalling, Vol. 11, Issue 12, pp. 871-83, 2000 (PubMed).

Vaughn, Wolford, Prochazka et al.: "Genomic structure and expression of human KCNJ9 (Kir3.3/GIRK3)." in: Biochemical and biophysical research communications, Vol. 274, Issue 2, pp. 302-9, 2000 (PubMed).

Gregory, Barlow, McLay et al.: "The DNA sequence and biological annotation of human chromosome 1." in: Nature, Vol. 441, Issue 7091, pp. 315-21, 2006 (PubMed).