Potassium Channel Subfamily K Member 1 (KCNK1) (N-Term) antibody

Antigen: Potassium Channel Subfamily K Member 1 (KCNK1)

Synonyms: TWIK-1, AI788889, DPK, HOHO, KCNO1, TWIK1, K2p1.1

Epitope: N-Term

Clonality: Polyclonal

Host: Rabbit

Application: Immunohistochemistry (IHC), Western Blotting (WB)

Catalog no.: ABIN350403

Additional Information

Alternative name: KCNK1 (TWIK-1) (n-terminal region)

Swiss-Prot: O08581

Immunogen: A synthetic peptide from the n-terminal region of mouse KCNK1 (TWIK-1) conjugated to an immunogenic carrier protein was used as the immunogen. The antigen is homologous in human and rat.

Format: Lyophilized

Description: Function: Weak inwardly rectifying potassium channel. Inhibited by quinine, barium, and internal acidification. Activated by protein kinase C. Tissue specificity: Widely expressed. Highest expression in brain, kidney, thyroid, salivary gland, adrenal gland, prostate, epididymis, uterus, placenta, colon and jejunum. Moderate expression in eyes, pituitary, pancreas, smooth muscle, testis and ovary. Very low levels in lung, aorta, liver, heart, skeletal muscle, thymus and spleen. In the brain, highest expression in cerebellar granule cells, brainstem, hippocampus and cerebral cortex. Subcellular location: Membrane, Multi-pass membrane protein. Also known as: Potassium channel subfamily K member 1, Inward rectifying potassium channel protein TWIK-1.

Specificity: Appears to be specific for KCNK1.

Application Details

Application Notes: IHC, WB. A dilution of 1 : 300 to 1 : 2000 is recommended. The optimal dilution should be determined by the end user. Not yet tested in other applications.

Purity: whole serum

Storage: Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.

Restrictions: For Research Use only

Publications

Publications: Lesage, Lauritzen, Duprat et al.: "The structure, function and distribution of the mouse TWIK-1 K+ channel." in: FEBS letters, Vol. 402, Issue 1, pp. 28-32, 1997 (PubMed).

Orias, Veluazquez, Tung et al.: "Cloning and localization of a double-pore K channel, KCNK1: exclusive expression in distal nephron segments." in: The American journal of physiology, Vol. 273, Issue 4 Pt 2, pp. F663-6, 1997 (PubMed).

Arrighi, Lesage, Scimeca et al.: "Structure, chromosome localization, and tissue distribution of the mouse twik K+ channel gene." in: FEBS letters, Vol. 425, Issue 2, pp. 310-6, 1998 (PubMed).

Gregory, Barlow, McLay et al.: "The DNA sequence and biological annotation of human chromosome 1." in: Nature, Vol. 441, Issue 7091, pp. 315-21, 2006 (PubMed).

Munton, Tweedie-Cullen, Livingstone-Zatchej et al.: "Qualitative and quantitative analyses of protein phosphorylation in naive and stimulated mouse synaptosomal preparations." in: Molecular & cellular proteomics : MCP, Vol. 6, Issue 2, pp. 283-93, 2007 (PubMed).