Potassium Large Conductance Calcium-Activated Channel, Subfamily M, alpha Member 1 (KCNMA1) (C-Term) antibody

Antigen: Potassium Large Conductance Calcium-Activated Channel, Subfamily M, alpha Member 1 (KCNMA1)

Synonyms: SLO, BKTM, SLO1, MaxiK, SAKCA, mSLO1, KCa1.1, MGC71881, SLO-ALPHA, bA205K10.1, DKFZp686K1437, RBSLO1, KCNMA, KCNMA1, Slo, Slo1, mSlo, mSlo1, Kcnma, KCNMA1b, KCNMA1c, kcnma1-A, kcnma1, si:ch211-39f22.2

Epitope: C-Term

Clonality: Polyclonal

Host: Rabbit

Application: Immunohistochemistry (IHC), Western Blotting (WB)

Catalog no.: ABIN350418

Additional Information

Alternative name: KCNMA1 (KCa1.1, SLO) (c-terminal region)

Swiss-Prot: Q12791

Immunogen: A synthetic peptide from 4th cytoplasmic domain of human Potassium channel subfamily M subunit alpha 1 (KCNMA1, KCa1.1, SLO) conjugated to an immunogenic carrier protein was used as the immunogen. The antigen shares 95% identity with rat and mouse sequence.

Format: Lyophilized

Description: Function: Potassium channel activated by both membrane depolarization or increase in cytosolic Ca(2+) that mediates export of K(+). It is also activated by the concentration of cytosolic Mg(2+). Its activation dampens the excitatory events that elevate the cytosolic Ca(2+) concentration and/or depolarize the cell membrane. It therefore contributes to repolarization of the membrane potential. Plays a key role in controlling excitability in a number of systems, such as regulation of the contraction of smooth muscle, the tuning of hair cells in the cochlea, regulation of transmitter release, and innate immunity. In smooth muscles, its activation by high level of Ca(2+), caused by ryanodine receptors in the sarcoplasmic reticulum, regulates the membrane potential. In cochlea cells, its number and kinetic properties partly determine the characteristic frequency of each hair cell and thereby helps to establish a tonotopic map. Kinetics of KCNMA1 channels are determined by alternative splicing, phosphorylation status and its combination with modulating beta subunits. Highly sensitive to both iberiotoxin (IbTx) and charybdotoxin (CTX). The protein was initially thought to contain two functionally distinct parts: The core channel (from the N-terminus to the S9 segment) that mediates the channel activity, and the cytoplasmic tail (from the S9 segment to the C-terminus) that mediates the calcium sensing. The situation is however more complex, since the core channel also contains binding sites for Ca(2+) and Mg(2+). Subcellular location: Membrane, Multi-pass membrane protein. Tissue specificity: Widely expressed. Except in myocytes, it is almost ubiquitously expressed. Also known as: Potassium channel subfamily M subunit alpha 1, Calcium-activated potassium channel subunit alpha-1, Calcium-activated potassium channel, subfamily M subunit alpha-1, Maxi K channel, MaxiK, BK channel, K(VCA)alpha, BKCA alpha, KCa1.1, Slowpoke homolog, Slo homolog, Slo-alpha, Slo1, hSlo.

Specificity: Appears to be specific for KCNMA1.

Application Details

Application Notes: IHC, WB (confirmed by recombinant protein). A dilution of 1 : 300 to 1 : 2000 is recommended. The optimal dilution should be determined by the end user. Not yet tested in other applications.

Purity: whole serum

Storage: Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.

Restrictions: For Research Use only

Publications

Publications: McCobb, Fowler, Featherstone et al.: "A human calcium-activated potassium channel gene expressed in vascular smooth muscle." in: The American journal of physiology, Vol. 269, Issue 3 Pt 2, pp. H767-77, 1995 (PubMed).

Dworetzky, Trojnacki, Gribkoff: "Cloning and expression of a human large-conductance calcium-activated potassium channel." in: Brain research. Molecular brain research, Vol. 27, Issue 1, pp. 189-93, 1995 (PubMed).

Tseng-Crank, Foster, Krause et al.: "Cloning, expression, and distribution of functionally distinct Ca(2+)-activated K+ channel isoforms from human brain." in: Neuron, Vol. 13, Issue 6, pp. 1315-30, 1995 (PubMed).

Deloukas, Earthrowl, Grafham et al.: "The DNA sequence and comparative analysis of human chromosome 10." in: Nature, Vol. 429, Issue 6990, pp. 375-81, 2004 (PubMed).

Du, Bautista, Yang et al.: "Calcium-sensitive potassium channelopathy in human epilepsy and paroxysmal movement disorder." in: Nature genetics, Vol. 37, Issue 7, pp. 733-8, 2005 (PubMed).