Potassium Large Conductance Calcium-Activated Channel, Subfamily M beta Member 3 (KCNMB3) (N-Term) antibody

Antigen: Potassium Large Conductance Calcium-Activated Channel, Subfamily M beta Member 3 (KCNMB3)

Synonyms: HBETA3, KCNMB2, KCNMBL, BKBETA3, SLOBETA3, KCNMB3, MGC138124

Epitope: N-Term

Clonality: Polyclonal

Host: Rabbit

Application: Immunohistochemistry (IHC), Western Blotting (WB)

Catalog no.: ABIN350421

Additional Information

Alternative name: KCNMB3 (Maxi K channel subunit beta-3) (n-terminal region)

Swiss-Prot: Q9NPA1

Immunogen: A synthetic peptide from the n-terminal region of human KCNMB3 (Maxi K channel subunit beta-3) conjugated to an immunogenic carrier protein was used as the immunogen. The antigen is homologous in rat and mouse.

Format: Lyophilized

Description: Function: Regulatory subunit of the calcium activated potassium KCNMA1 (maxiK) channel. Modulates the calcium sensitivity and gating kinetics of KCNMA1, thereby contributing to KCNMA1 channel diversity. Alters the functional properties of the current expressed by the KCNMA1 channel. Isoform, isoform  and isoform  partially inactivate the current of KCNBMA. Isoform induces a fast and incomplete inactivation of KCNMA1 channel that is detectable only at large depolarizations. In contrast, isoform does not induce detectable inactivation of KCNMA1. Two or more subunits of KCNMB3 are required to block the KCNMA1 tetramer. Tissue specificity: Isoform, isoform and isoform are widely expressed. Isoform is expressed placenta, pancreas, kidney and heart. Isoform<||a href="http://www.uniprot.org/uniprot/Q9NPA1#Q9NPA1-1"> and isoform are highly expressed in pancreas and testis. Subcellular location: Membrane, Multi-pass membrane protein.<||/a> Also known as: Calcium-activated potassium channel subunit beta-3, Calcium-activated potassium channel, subfamily M subunit beta-3, Maxi K channel subunit beta-3, BK channel subunit beta-3, BKbeta3, Hbeta3, K(VCA)beta-3, Slo-beta-3, Charybdotoxin receptor subunit beta-3, KCNMBL.

Specificity: Appears to be specific for KCNMB3.

Application Details

Application Notes: IHC, WB. A dilution of 1 : 300 to 1 : 2000 is recommended. The optimal dilution should be determined by the end user. Not yet tested in other applications.

Purity: whole serum

Storage: Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.

Restrictions: For Research Use only

Publications

Publications: Riazi, Brinkman-Mills, Johnson et al.: "Identification of a putative regulatory subunit of a calcium-activated potassium channel in the dup(3q) syndrome region and a related sequence on 22q11.2." in: Genomics, Vol. 62, Issue 1, pp. 90-4, 2000 (PubMed).

Uebele, Lagrutta, Wade et al.: "Cloning and functional expression of two families of beta-subunits of the large conductance calcium-activated K+ channel." in: The Journal of biological chemistry, Vol. 275, Issue 30, pp. 23211-8, 2000 (PubMed).

Behrens, Nolting, Reimann et al.: "hKCNMB3 and hKCNMB4, cloning and characterization of two members of the large-conductance calcium-activated potassium channel beta subunit family." in: FEBS letters, Vol. 474, Issue 1, pp. 99-106, 2000 (PubMed).

Zeng, Xia, Lingle: "Redox-sensitive extracellular gates formed by auxiliary beta subunits of calcium-activated potassium channels." in: Nature structural biology, Vol. 10, Issue 6, pp. 448-54, 2003 (PubMed).