Potassium Channel, Subfamily T, Member 1 (KCNT1) (4th Cytoplasmic Domain) antibody

Antigen: Potassium Channel, Subfamily T, Member 1 (KCNT1)

Synonyms: SLACK, KCa4.1, FLJ41282, bA100C15.2, Slack, rSlo2, slo2, mKIAA1422, C030030G16Rik, slack, Slo2.2, MGC146594, MGC172997

Binding Site: 4th Cytoplasmic Domain

Clonality: Polyclonal

Host: Rabbit

Reactivity: Please enquire

Application: Immunohistochemistry (IHC), Western Blotting (WB)

Catalog no.: ABIN350427

Additional Information

Alternative name: KCNT1 (KCa4.1)

UniProt: Q5JUK3

Immunogen: A synthetic peptide from the 4th cytoplasmic domain of human Potassium channel subfamily T member 1 (KCNT1, KCa4.1) conjugated to an immunogenic carrier protein was used as the immunogen. The antigen is homologous in monkey.

Format: Lyophilized

Description: FUNCTION: Testis-specific potassium channel activated by both intracellular pH and membrane voltage that mediates export of K+. May be involved in sperm capacitation and/or the acrosome reaction, essential steps in fertilization where changes in both intracellular pH and membrane potential are known to occur. In contrast to KCNMA1/SLO1, it is not activated by Ca2+ or Mg2+. Subcellular location: Membrane; Multi-pass membrane protein Tissue specificity: Testis-specific. Mainly expressed in spermatocytes. Detected in brain and brainstem, in vestibular and oculomotor nuclei, the medial nucleus of the trapezoid in the auditory system, inolfactory bulb, red nucleus, and deep cerebellar nuclei. Detected in thalamus, substantia nigra, and amygdala (at protein level). Highly expressed in the brain and kidney. Highest expression in liver, brain and spinal cord. Lowest expression in skeletal muscle. Also known as: Potassium channel subfamily T member 1, KCa4.1, SLACK.

Specificity: Appears to be specific for KCNT1.

Application Details

Application Notes: IHC, WB (confirmed by recombinant protein). A dilution of 1 : 300 to 1 : 2000 is recommended. The optimal dilution should be determined by the end user. Not yet tested in other applications.

Purity: whole serum

Storage: Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.

Restrictions: For Research Use only

Publications

Nagase, Kikuno, Ishikawa et al.: "Prediction of the coding sequences of unidentified human genes. XVI. The complete sequences of 150 new cDNA clones from brain which code for large proteins in vitro." in: DNA research : an international journal for rapid publication of reports on genes and genomes, Vol. 7, Issue 1, pp. 65-73, 2000 (PubMed).

Humphray, Oliver, Hunt et al.: "DNA sequence and analysis of human chromosome 9." in: Nature, Vol. 429, Issue 6990, pp. 369-74, 2004 (PubMed).