KiSS-1 Metastasis-Suppressor (KISS1) (N-Term) antibody

Antigen: KiSS-1 Metastasis-Suppressor (KISS1)

Synonyms: KiSS-1, METASTIN, MGC39258

Epitope: N-Term

Clonality: Polyclonal

Host: Rabbit

Application: Immunohistochemistry (IHC), Western Blotting (WB)

Catalog no.: ABIN350429

Additional Information

Alternative name: Kisspeptin (Metastasis suppressor KiSS 1) (n-terminal region)

Swiss-Prot: Q15726

Immunogen: A synthetic peptide from n-terminal region of human Kisspeptin (Metastasis suppressor KiSS 1) conjugated to an immunogenic carrier protein was used as the immunogen.

Format: Lyophilized

Description: FUNCTION: Metastasis suppressor protein in malignant melanomas and in some breast cancers. May regulate events downstream of cell-matrix adhesion, perhaps involving cytoskeletal reorganization. Generates a C-terminally amidated peptide, metastin which functions as the endogenous ligand of the G-protein coupled receptor GPR54. Activation of the receptor inhibits cell proliferation and cell migration, key characteristics of tumor metastasis. Kp-10 is a decapeptide derived from the primary translation product, isolated in conditioned medium of first trimester trophoblast. Kp-10, but not other kisspeptins, increased intracellular Ca2+ levels in isolated first trimester trophoblasts. Kp-10 is a paracrine/endocrine regulator in fine-tuning trophoblast invasion generated by the trophoblast itself. The receptor is also essential for normal gonadotropin-released hormone physiology and for puberty. The hypothalamic KiSS1/GPR54 system is a pivotal factor in central regulation of the gonadotropic axis at puberty and in adulthood. SUBCELLULAR LOCATION: Secreted Tissue specificity: Very high expression in placenta, with the next highest level in testis and moderate levels in pancreas, liver, small intestine and brain at much lower levels. Expression levels increased in both early placentas and molar pregnancies and are reduced in choriocarcinoma cells. Expressed at higher levels in first trimester trophoblasts than at term of gestation, but only expressed in the villous trophoblast. Also known as: Metastasis-suppressor KiSS-1, Kisspeptin-1, Metastin, KISS1.

Specificity: Appears to be specific for Kisspeptin.

Application Details

Application Notes: IHC, WB. A dilution of 1 : 300 to 1 : 2000 is recommended. The optimal dilution should be determined by the end user. Not tested in other applications.

Purity: whole serum

Storage: Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.

Restrictions: For Research Use only

Publications

Publications: Lee, Miele, Hicks et al.: "KiSS-1, a novel human malignant melanoma metastasis-suppressor gene." in: Journal of the National Cancer Institute, Vol. 88, Issue 23, pp. 1731-7, 1996 (PubMed).

West, Vojta, Welch et al.: "Chromosome localization and genomic structure of the KiSS-1 metastasis suppressor gene (KISS1)." in: Genomics, Vol. 54, Issue 1, pp. 145-8, 1999 (PubMed).

Wan, Gong, Qin et al.: "Large-scale cDNA transfection screening for genes related to cancer development and progression." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 101, Issue 44, pp. 15724-9, 2004 (PubMed).

Semple, Achermann, Ellery et al.: "Two novel missense mutations in g protein-coupled receptor 54 in a patient with hypogonadotropic hypogonadism." in: The Journal of clinical endocrinology and metabolism, Vol. 90, Issue 3, pp. 1849-55, 2005 (PubMed).

Gregory, Barlow, McLay et al.: "The DNA sequence and biological annotation of human chromosome 1." in: Nature, Vol. 441, Issue 7091, pp. 315-21, 2006 (PubMed).