Kruppel-Like Factor 1/2/4/5/7/12 (KLF1/2/4/5/7/12) (C-Term) antibody

Antigen: Kruppel-Like Factor 1/2/4/5/7/12 (KLF1/2/4/5/7/12)

Synonyms: CKLF, IKLF, BTEB2, Bteb2, 4930520J07Rik

Epitope: C-Term

Clonality: Polyclonal

Host: Rabbit

Application: Immunohistochemistry (IHC), Western Blotting (WB)

Catalog no.: ABIN350431

Additional Information

Alternative name: Krueppel-like factor 1, 2, 4, 5, 7, 12 (KLF1, KLF2, KLF4, KLF5, KLF7, KLF12) (c-terminal)

Swiss-Prot: Q13887

Immunogen: A synthetic from the c-terminal of human Krueppel-like factor 1, 2, 4, 5, 7, 12 (KLF1, KLF2, KLF4, KLF5, KLF7, KLF12) conjugated to an immunogenic carrier protein was used as the immunogen. The antigen is homologous in many species including rat, mouse.

Format: Lyophilized

Description: Belongs to the krueppel C2H2-type zinc-finger protein family. Also known as: Krueppel-like factor 1 (KLF1, Erythroid transcription factor), Krueppel-like factor 2 (KLF2, Lung krueppel-like factor), Krueppel-like factor 4 (KLF4, Epithelial zinc finger protein EZF, Gut-enriched krueppel-like factor, EZF, GKLF), Krueppel-like factor 5 (KLF5, Intestinal-enriched krueppel-like factor, Colon krueppel-like factor, Transcription factor BTEB2, Basic transcription element-binding protein 2, BTE-binding protein 2, GC-box-binding protein 2), Krueppel-like factor 7 (KLF7), Krueppel-like factor 12 (KLF12, AP2REP, HSPC122).

Specificity: Appears to be specific for Krueppel-like factor 1, 2, 4, 5, 7, 12 (KLF1, KLF2, KLF4, KLF5, KLF7, KLF12).

Application Details

Application Notes: IHC, WB. A dilution of 1 : 300 to 1 : 2000 is recommended. The optimal dilution should be determined by the end user. Not yet tested in other applications.

Purity: whole serum

Storage: Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.

Restrictions: For Research Use only

Publications

Publications: Anderson, Kern, Crable et al.: "Isolation of a gene encoding a functional zinc finger protein homologous to erythroid Krüppel-like factor: identification of a new multigene family." in: Molecular and cellular biology, Vol. 15, Issue 11, pp. 5957-65, 1995 (PubMed).

Shields, Christy, Yang: "Identification and characterization of a gene encoding a gut-enriched Krüppel-like factor expressed during growth arrest." in: The Journal of biological chemistry, Vol. 271, Issue 33, pp. 20009-17, 1996 (PubMed).

Matsumoto, Laub, Aldabe et al.: "Cloning the cDNA for a new human zinc finger protein defines a group of closely related Krüppel-like transcription factors." in: The Journal of biological chemistry, Vol. 273, Issue 43, pp. 28229-37, 1998 (PubMed).

Ohnishi, Laub, Matsumoto et al.: "Developmental expression of the mouse gene coding for the Krüppel-like transcription factor KLF5." in: Developmental dynamics : an official publication of the American Association of Anatomists, Vol. 217, Issue 4, pp. 421-9, 2000 (PubMed).

Siatecka, Xue, Bieker: "Sumoylation of EKLF promotes transcriptional repression and is involved in inhibition of megakaryopoiesis." in: Molecular and cellular biology, Vol. 27, Issue 24, pp. 8547-60, 2007 (PubMed).