Large Tumor Suppressor 2 (LATS2) (N-Term) antibody

Antigen: Large Tumor Suppressor 2 (LATS2)

Synonyms: LATS2, lats2, MGC122292, AV277261, AW228608, 4932411G09Rik

Epitope: N-Term

Clonality: Polyclonal

Host: Rabbit

Application: Immunohistochemistry (IHC), Western Blotting (WB)

Catalog no.: ABIN350433

Additional Information

Alternative name: LATS2 (n-terminal)

Swiss-Prot: Q7TSJ6

Immunogen: A synthetic peptide to the n-terminal of mouse LATS2 conjugated to an immunogenic carrier protein was used as the immunogen.

Format: Lyophilized

Description: FUNCTION: Tumor suppressor which plays a critical role in centrosome duplication, maintenance of mitotic fidelity and genomic stability. Negatively regulates G1/S transition by down-regulating cyclin E/CDK2 kinase activity. Negative regulator of the androgen receptor. Subcellular location: Centrosome. Cytoplasm. Note: Co-localizes with STK6 at the centrosomes during interphase, early prophase and cytokinesis. Migrates to the spindle poles during mitosis, and to the midbody during cytokinesis. Tissue specificity: Expressed at high levels in ovary and testis and at lower levels in all other tissues examined. Also known as: Serine/threonine-protein kinase LATS2, Large tumor suppressor homolog 2, Serine/threonine-protein kinase kpm, Kinase phosphorylated during mitosis protein.

Specificity: Appears to be specific for LATS2.

Application Details

Application Notes: IHC, WB. A dilution of 1: 300 to 1: 2000 is recommended. The optimal dilution should be determined by the end user. Not yet tested in other applications.

Purity: whole serum

Storage: Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.

Restrictions: For Research Use only

Publications

Publications: Yabuta, Fujii, Copeland et al.: "Structure, expression, and chromosome mapping of LATS2, a mammalian homologue of the Drosophila tumor suppressor gene lats/warts." in: Genomics, Vol. 63, Issue 2, pp. 263-70, 2000 (PubMed).

Ota, Suzuki, Nishikawa et al.: "Complete sequencing and characterization of 21,243 full-length human cDNAs." in: Nature genetics, Vol. 36, Issue 1, pp. 40-5, 2003 (PubMed).

McPherson, Tamblyn, Elia et al.: "Lats2/Kpm is required for embryonic development, proliferation control and genomic integrity." in: The EMBO journal, Vol. 23, Issue 18, pp. 3677-88, 2004 (PubMed).

Carninci, Kasukawa, Katayama et al.: "The transcriptional landscape of the mammalian genome." in: Science (New York, N.Y.), Vol. 309, Issue 5740, pp. 1559-63, 2005 (PubMed).

Dephoure, Zhou, Villuen et al.: "A quantitative atlas of mitotic phosphorylation." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 105, Issue 31, pp. 10762-7, 2008 (PubMed).