Mas-Related G-Protein Coupled Receptor Member D (MRGPRD) (4th cytoplasmic loop) antibody

Antigen: Mas-Related G-Protein Coupled Receptor Member D (MRGPRD)

Synonyms: MRGD, TGR7

Epitope: 4th cytoplasmic loop

Clonality: Polyclonal

Host: Rabbit

Reactivity: Mouse (Murine)

Application: Immunohistochemistry (IHC), Western Blotting (WB)

Catalog no.: ABIN350442

Additional Information

Alternative name: Mas-related G-protein coupled receptor member D (MRGPRD) (4th cytoplasmic loop)

Swiss-Prot: Q91ZB8

Immunogen: A synthetic peptide from the 4th cytoplasmic loop of mouse Mas-related G-protein coupled receptor member D (MRGPRD) conjugated to an immunogenic carrier protein was used as the immunogen. The antigen shares 81% identity with rat's sequence.

Format: Lyophilized

Description: FUNCTION: May regulate nociceptor function and/or development, including the sensation or modulation of pain. Functions as a specific membrane receptor for beta-alanine. Beta-alanine at micromolar doses specifically evoked Ca(2+) influx in cells expressing the receptor. Beta-alanine decreases forskolin-stimulated cAMP production in cells expressing the receptor, suggesting that the receptor couples with G-protein G(q) and G(i). SUBCELLULAR LOCATION: Cell membrane; Multi-pass membrane protein. Note: Localized at the plasma membrane but internalized into the cytoplasm after treatment with beta-alanine. Also known as: Mas-related G-protein coupled receptor, member D, Beta-alanine receptor, G-protein coupled receptor TGR7, MRGD, MRGPRD.

Specificity: Appears to be specific for MRGPRD.

Application Details

Application Notes: IHC, WB. A dilution of 1 : 300 to 1 : 2000 is recommended. The optimal dilution should be determined by the end user. Not yet tested in other applications.

Purity: whole serum

Storage: Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.

Restrictions: For Research Use only

Publications

Publications: Dong, Han, Zylka et al.: "A diverse family of GPCRs expressed in specific subsets of nociceptive sensory neurons." in: Cell, Vol. 106, Issue 5, pp. 619-32, 2001 (PubMed).

Shinohara, Harada, Ogi et al.: "Identification of a G protein-coupled receptor specifically responsive to beta-alanine." in: The Journal of biological chemistry, Vol. 279, Issue 22, pp. 23559-64, 2004 (PubMed).