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Mature NT3 (AA 140-200) antibody

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AA 140-200
Human, Rat (Rattus), Mouse (Murine)
(1), (1), (1)
Immunohistochemistry (IHC), Western Blotting (WB)
(1), (1)
Pubmed 5 references available
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Quantity 100 μL
Shipping to United States ( )
Availability Will be delivered in 7 to 8 Business Days
Immunogen A synthetic peptide from aa region 140-200 of human NT3 conjugated to an immunogenic carrier protein was used as the antigen. The peptide is homologous in many other species including rat and mouse.
Specificity Specific for mature NT-3.
Purification Whole serum
Background Function: Seems to promotes the survival of visceral and proprioceptive sensory neurons.
Tissue specificity: Brain and peripheral tissues.
Subcellular location: Secreted. Also known as: Neurotrophin-3, HDNF, Nerve growth factor 2, NGF-2, NTF3.
Application Notes A dilution of 1 : 300 to 1 : 2000 is recommended.
The optimal dilution should be determined by the end user.
Not yet tested in other applications.
Restrictions For Research Use only
Format Lyophilized
Reconstitution Reconstitute in 100 µL of sterile water. Centrifuge to remove any insoluble material.
Handling Advice Avoid freeze and thaw cycles.
Storage 4 °C/-20 °C
Storage Comment Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.
Expiry Date 12 months
Background publications Robinson, Radziejewski, Stuart et al.: "Structure of the brain-derived neurotrophic factor/neurotrophin 3 heterodimer." in: Biochemistry, Vol. 34, Issue 13, pp. 4139-46, 1995 (PubMed).

Kaisho, Yoshimura, Nakahama: "Cloning and expression of a cDNA encoding a novel human neurotrophic factor." in: FEBS letters, Vol. 266, Issue 1-2, pp. 187-91, 1990 (PubMed).

Rosenthal, Goeddel, Nguyen et al.: "Primary structure and biological activity of a novel human neurotrophic factor." in: Neuron, Vol. 4, Issue 5, pp. 767-73, 1990 (PubMed).

Jones, Reichardt: "Molecular cloning of a human gene that is a member of the nerve growth factor family." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 87, Issue 20, pp. 8060-4, 1990 (PubMed).

Hallboeoek, Ibuaunez, Persson: "Evolutionary studies of the nerve growth factor family reveal a novel member abundantly expressed in Xenopus ovary." in: Neuron, Vol. 6, Issue 5, pp. 845-58, 1991 (PubMed).

Catalog No. ABIN350457
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