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Opsin 4 (OPN4) antibody
|Synonyms||Gm533, 1110007J02Rik, MOP, MGC142118, opn4, OPN4, OPN4-2, opn4-A, Xmop, opn4d|
Alternatives Immunohistochemistry (IHC), Western Blotting (WB)
|5 references available|
|Quantity||100 µl (Variants)|
|Price||454.67 $ Plus shipping costs $45.00|
|Availability||Will be delivered in 7 to 8 Business Days|
|Alternative name||Melanopsin (OPN4, Opsin-4, MOP)|
|Immunogen||A synthetic peptide as a part of human opsin-4 (OPN4, melanopsin, MOP) conjugated to immunogenic carrier protein was used as the immunogen.|
|Description||Opsins are members of the guanine nucleotide-binding protein (G protein)-coupled receptor superfamily. This gene encodes a photoreceptive opsin protein that is expressed within the ganglion and amacrine cell layers of the retina. In mouse, retinal ganglion cell axons expressing this gene projected to the suprachiasmatic nucleus and other brain nuclei involved in circadian photoentrainment. In mouse, this protein is coupled to a transient receptor potential (TRP) ion channel through a G protein signaling pathway and produces a physiologic light response via membrane depolarization and increased intracellular calcium. The protein functions as a sensory photopigment and may also have photoisomerase activity. Experiments with knockout mice indicate that this gene attenuates, but does not abolish, photoentrainment. Alternative splicing results in multiple transcript variants encoding different isoforms. FUNCTION: Photoreceptor required for regulation of circadian rhythm. Contributes to pupillar reflex and other non-image forming responses to light. May be able to isomerize covalently bound all-trans retinal back to 11-cis retinal. SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein. Note: Found in soma, dendrites and proximal part of axons of certain retinal ganglion cells . TISSUE SPECIFICITY: Eye. Expression is restricted within the ganglion and amacrine cell layers of the retina. Also known as: Melanopsin, opsin-4, OPN4, MOP, OPN 4, MGC142118.|
|Specificity||Appears to be specific for opsin-4.|
|Application Notes||IHC, WB. A dilution of 1 : 300 to 1 : 2000 is recommended. The optimal dilution should be determined by the end user. Not yet tested in other applications.|
|Purification||The antibody is affinity-purified from rabbit antiserum by affinity chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide is removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.|
|Storage||Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.|
|Restrictions||For Research Use only|
Provencio, Rodriguez, Jiang et al.: "A novel human opsin in the inner retina." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 20, Issue 2, pp. 600-5, 2000 (PubMed).
Qiu, Kumbalasiri, Carlson et al.: "Induction of photosensitivity by heterologous expression of melanopsin." in: Nature, Vol. 433, Issue 7027, pp. 745-9, 2005 (PubMed).
Melyan, Tarttelin, Bellingham et al.: "Addition of human melanopsin renders mammalian cells photoresponsive." in: Nature, Vol. 433, Issue 7027, pp. 741-5, 2005 (PubMed).
Dacey, Liao, Peterson et al.: "Melanopsin-expressing ganglion cells in primate retina signal colour and irradiance and project to the LGN." in: Nature, Vol. 433, Issue 7027, pp. 749-54, 2005 (PubMed).
Kumbalasiri, Rollag, Isoldi et al.: "Melanopsin triggers the release of internal calcium stores in response to light." in: Photochemistry and photobiology, Vol. 83, Issue 2, pp. 273-9, 2007 (PubMed).
|Reactivities||Human (8), Mouse (Murine) (3), Rat (Rattus) (2)|
|Applications||Western Blotting (WB) (11), ELISA (6), Immunofluorescence (IF) (4), Immunocytochemistry (ICC) (3), Immunohistochemistry (Frozen Sections) (IHC (fro)) (2), Immunohistochemistry (IHC) (2), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (2)|