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Microtubule-Associated Protein 1 Light Chain 3 alpha (MAP1LC3A) antibody

Details for Product No. ABIN350464, Supplier: Log in to see
Antigen
  • MGC69006
  • MGC89867
  • MAP1LC3A
  • map1lc3a
  • Anx3
  • LC3
  • LRRGT00047
  • ATG8E
  • LC3A
  • MAP1ALC3
  • MAP1BLC3
  • 1010001H21Rik
  • 4922501H04Rik
  • LC3a
  • zgc:77094
  • Map1lc3
  • zgc:56434
  • wu:fb60g11
  • Lc3
  • b3gnt5
  • fb12f12
  • wu:fb12f12
  • wu:fe50g08
  • microtubule-associated protein 1 light chain 3 alpha
  • annexin A3
  • microtubule-associated protein 1 light chain 3 beta
  • UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyltransferase 5a
  • map1lc3a
  • MAP1LC3A
  • Anxa3
  • Map1lc3a
  • map1lc3b
  • b3gnt5a
Reactivity
Human, Mouse (Murine), Rat (Rattus)
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189
161
35
25
17
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12
8
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4
3
3
2
1
1
1
1
Host
Rabbit
336
35
5
4
Clonality
Polyclonal
Conjugate
Un-conjugated
25
23
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20
17
17
4
4
4
3
3
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2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
Application
Immunohistochemistry (IHC), Western Blotting (WB)
310
162
134
106
70
61
31
28
17
15
14
10
6
3
2
1
Options
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Immunogen A synthetic peptide from human microtubule-associated proteins 1A/1B light chain 3A (MAP1LC3A, MAP1A/MAP1B LC3 A) conjugated to an immunogenic carrier protein has been used as the immunogen. The antigen is homologous in rat, mouse and bovine.
Specificity Specific for MAP1LC3A.
Purification Whole serum
Alternative Name MAP1LC3A (MAP1LC3A Antibody Abstract)
Background MAP1A and MAP1B are microtubule-associated proteins which mediate the physical interactions between microtubules and components of the cytoskeleton. MAP1A and MAP1B each consist of a heavy chain subunit and multiple light chain subunits. The protein encoded by this gene is one of the light chain subunits and can associate with either MAP1A or MAP1B. Two transcript variants encoding different isoforms have been found for this gene. This protein is probably involved in formation of autophagosomal vacuoles.
Subcellular location: Cytoplasm. Intracytoplasmic membrane, Lipid-anchor. Cytoplasmic vesicle, autophagosome membrane, Lipid-anchor. Note: LC3-II binds to the autophagic membranes.
Tissue specificity: Most abundant in heart, brain, liver, skeletal muscle and testis but absent in thymus and peripheral blood leukocytes. Also known as: MAP1A/MAP1B LC3 A, MAP1A/1B light chain 3 A, MAP1 light chain 3-like protein 1, Microtubule-associated protein 1 light chain 3 alpha,autophagy-related protein LC3 Aautophagy-related ubiquitin-like modifier LC3 A, MAP1ALC3, MAP1BLC3, MAP1LC3A.
Research Area Cancer, Autophagy, Cytoskeleton, Proteolysis / Ubiquitin
Application Notes Use at a dilution of 1 : 200 to 1 : 2000.
The optimal dilution should be determined by the end user.
Not yet tested in other applications.
Restrictions For Research Use only
Format Lyophilized
Reconstitution Reconstitute in 150 µL of sterile water. Centrifuge to remove any insoluble material.
Handling Advice Avoid freeze and thaw cycles.
Storage 4 °C/-20 °C
Storage Comment Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.
Expiry Date 12 months
Supplier Images
 image for anti-Microtubule-Associated Protein 1 Light Chain 3 alpha (MAP1LC3A) antibody (ABIN350464) WB on human neutrophil lysate using Rabbit antibody to microtubule-associated protein...
Background publications Luenemann, Schmidt, Dalakas, Muenz: "Macroautophagy as a pathomechanism in sporadic inclusion body myositis." in: Autophagy, Vol. 3, Issue 4, pp. 384-6, 2007 (PubMed).

Pankiv, Clausen, Lamark, Brech, Bruun, Outzen, a: "p62/SQSTM1 binds directly to Atg8/LC3 to facilitate degradation of ubiquitinated protein aggregates by autophagy." in: The Journal of biological chemistry, Vol. 282, Issue 33, pp. 24131-45, 2007 (PubMed).

Mehrle, Rosenfelder, Schupp, del Val, Arlt, Hahne, Bechtel, Simpson, Hofmann, Hide, Glatting, Huber, Pepperkok, Poustka, Wiemann: "The LIFEdb database in 2006." in: Nucleic acids research, Vol. 34, Issue Database issue, pp. D415-8, 2005 (PubMed).

Tanida, Ueno, Kominami: "Human light chain 3/MAP1LC3B is cleaved at its carboxyl-terminal Met121 to expose Gly120 for lipidation and targeting to autophagosomal membranes." in: The Journal of biological chemistry, Vol. 279, Issue 46, pp. 47704-10, 2004 (PubMed).

Deloukas, Matthews, Ashurst, Burton, Gilbert, Jones, Stavrides, Almeida, Babbage, Bagguley, Bailey, Barlow, Bates, Beard, Beare, Beasley, Bird, Blakey, Bridgeman, Brown, Buck, Burrill, Butler, Carder et al.: "The DNA sequence and comparative analysis of human chromosome 20. ..." in: Nature, Vol. 414, Issue 6866, pp. 865-71, 2002 (PubMed).