Microtubule-Associated Protein 1 Light Chain 3 alpha (MAP1LC3A) antibody

Antigen: Microtubule-Associated Protein 1 Light Chain 3 alpha (MAP1LC3A)

Synonyms: LC3, LC3A, MAP1ALC3, MAP1BLC3, LC3a, 1010001H21Rik, 4922501H04Rik, MGC105263, MGC69006, map1lc3a, MGC53333, zgc:77094, MAP1LC3A, MGC89867, MGC138125

Clonality: Polyclonal

Host: Rabbit

Reactivity: Please enquire

Conjugate: Un-conjugated

Application: Immunohistochemistry (IHC), Western Blotting (WB)

Catalog no.: ABIN350464

Additional Information

Alternative name: microtubule-associated protein 1 light chain 3alpha (MAP1LC3A, MAP1ALC3, MAP1BLC3, MAP1A/MAP1B LC3 A)

UniProt: Q9H492

Immunogen: A synthetic peptide from human microtubule-associated proteins 1A/1B light chain 3A (MAP1LC3A, MAP1A/MAP1B LC3 A) conjugated to an immunogenic carrier protein has been used as the immunogen. The antigen is homologous in rat, mouse and bovine.

Format: Lyophilized

Description: MAP1A and MAP1B are microtubule-associated proteins which mediate the physical interactions between microtubules and components of the cytoskeleton. MAP1A and MAP1B each consist of a heavy chain subunit and multiple light chain subunits. The protein encoded by this gene is one of the light chain subunits and can associate with either MAP1A or MAP1B. Two transcript variants encoding different isoforms have been found for this gene. This protein is probably involved in formation of autophagosomal vacuoles. SUBCELLULAR LOCATION: Cytoplasm. Intracytoplasmic membrane; Lipid-anchor. Cytoplasmic vesicle, autophagosome membrane; Lipid-anchor. Note: LC3-II binds to the autophagic membranes. TISSUE SPECIFICITY: Most abundant in heart, brain, liver, skeletal muscle and testis but absent in thymus and peripheral blood leukocytes. Also known as: MAP1A/MAP1B LC3 A, MAP1A/1B light chain 3 A, MAP1 light chain 3-like protein 1, Microtubule-associated protein 1 light chain 3 alpha,autophagy-related protein LC3 Aautophagy-related ubiquitin-like modifier LC3 A, MAP1ALC3, MAP1BLC3, MAP1LC3A.

Specificity: Appears to be specific for MAP1LC3A.

Application Details

Application Notes: IHC, WB. Use at a dilution of 1 : 200 to 1 : 2000. The optimal dilution should be determined by the end user. Not yet tested in other applications.

Purity: whole serum

Purification: The antibody is affinity-purified from rabbit antiserum by affinity chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide is removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

Storage: Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.

Research Area: Autophagy

Restrictions: For Research Use only

Images

WB on human neutrophil lysate using Rabbit antibody to microtubule-associated protein 1 light chain 3 alpha (MAP1LC3A, MAP1ALC3, MAP1BLC3, MAP1A/MAP1B LC3 A): whole serum (ABIN350464) at a dilution of 1 : 100. Pre-absorption of the antibody with the immunising peptide completely abolishes the detected band.

Publications

Deloukas, Matthews, Ashurst et al.: "The DNA sequence and comparative analysis of human chromosome 20." in: Nature, Vol. 414, Issue 6866, pp. 865-71, 2002 (PubMed).

Tanida, Ueno, Kominami: "Human light chain 3/MAP1LC3B is cleaved at its carboxyl-terminal Met121 to expose Gly120 for lipidation and targeting to autophagosomal membranes." in: The Journal of biological chemistry, Vol. 279, Issue 46, pp. 47704-10, 2004 (PubMed).

Mehrle, Rosenfelder, Schupp et al.: "The LIFEdb database in 2006." in: Nucleic acids research, Vol. 34, Issue Database issue, pp. D415-8, 2005 (PubMed).

Luenemann, Schmidt, Dalakas et al.: "Macroautophagy as a pathomechanism in sporadic inclusion body myositis." in: Autophagy, Vol. 3, Issue 4, pp. 384-6, 2007 (PubMed).

Pankiv, Clausen, Lamark et al.: "p62/SQSTM1 binds directly to Atg8/LC3 to facilitate degradation of ubiquitinated protein aggregates by autophagy." in: The Journal of biological chemistry, Vol. 282, Issue 33, pp. 24131-45, 2007 (PubMed).