Microtubule-Associated Protein 1 Light Chain 3 beta (MAP1LC3B) antibody

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Antigen
  • Map1lc3
  • zgc:56434
  • wu:fb60g11
  • map1lc3b
  • MGC76283
  • MAP1LC3B
  • ATG8F
  • LC3B
  • MAP1A/1BLC3
  • MAP1LC3B-a
  • 1010001C15Rik
  • Atg8
  • LC3b
  • Mpl3
  • zbs559
  • microtubule-associated protein 1 light chain 3 beta
  • map1lc3b
  • MAP1LC3B
  • LOC100361973
  • Map1lc3b
Alternatives
anti-Cow (Bovine) Microtubule-Associated Protein 1 Light Chain 3 beta antibody for Electron Microscopy
Reactivity
Human
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Host
Rabbit
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Clonality
Polyclonal
Conjugate
Un-conjugated
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Application
Immunohistochemistry (IHC), Western Blotting (WB)
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Options
Supplier
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Immunogen A synthetic peptide from human microtubule-associated proteins 1A/1B light chain 3B (MAP1A/MAP1B LC3 B, Map1lc3b) conjugated to an immunogenic carrier protein has been used as the antigen
Specificity Specific for MAP1LC3B.
Purification Whole serum
Alternative Name MAP1A/MAP1B LC3 B (MAP1LC3B Antibody Abstract)
Background Autophagy is the process by which endogenous proteins and damaged organelles are destroyed intracellularly.autophagy is postulated to be essential for cell homeostasis and cell remodeling during differentiation, metamorphosis, non-apoptotic cell death, and aging. Reduced levels ofautophagy have been described in some malignant tumors, and a role forautophagy in controlling the unregulated cell growth linked to cancer has been proposed. The product of this gene is a subunit of neuronal microtubule-associated MAP1A and MAP1B proteins, which are involved in microtubule assembly and important for neurogenesis. Studies on the rat homolog implicate a role for this gene inautophagy, a process that involves the bulk degradation of cytoplasmic component.
Function: Probably involved in formation of autophagosomal vacuoles (autophagosomes). May mediate MAPlA and MAPlB microtubule binding activity during development of the nervous system
Subunit: 3 different light chains, LC1, LC2 and LC3, can associate with MAP1A and MAP1B proteins.
Subcellular location: Cytoplasm. Intracytoplasmic membrane, Lipid-anchor. Cytoplasmic vesicle, autophagosome membrane, Lipid-anchor.Note: LC3-II binds to the autophagic membranes. Also known as: MLP3B, Map1alc3, Map1lc3, Microtubule-associated protein 1 light chain 3 beta, MAP1A/MAP1B LC3 B, MAP1A/1B light chain 3 B, MAP1 light chain 3-like protein 2,autophagy-related protein LC3 B,autophagy-related ubiquitin-like modifier LC3 B, Mpl3, MGC93422.
Pathways Autophagy
Application Notes Use at a dilution of 1 : 300 to 1 : 2000.
The optimal dilution should be determined by the end user.
Restrictions For Research Use only
Format Lyophilized
Reconstitution Reconstitute in 150 µL of sterile water. Centrifuge to remove any insoluble material.
Handling Advice Avoid freeze and thaw cycles.
Storage 4 °C/-20 °C
Storage Comment Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.
Expiry Date 12 months
Background publications Tanida, Ueno, Kominami: "Human light chain 3/MAP1LC3B is cleaved at its carboxyl-terminal Met121 to expose Gly120 for lipidation and targeting to autophagosomal membranes." in: The Journal of biological chemistry, Vol. 279, Issue 46, pp. 47704-10, 2004 (PubMed).

Wiemann, Arlt, Huber, Wellenreuther, Schleeger, Mehrle, Bechtel, Sauermann, Korf, Pepperkok, Sültmann, Poustka: "From ORFeome to biology: a functional genomics pipeline." in: Genome research, Vol. 14, Issue 10B, pp. 2136-44, 2004 (PubMed).

He, Dang, Dai, Guo, Wu, She, Pei, Chen, Ling, Wu, Zhao, Liu, Yu: "Post-translational modifications of three members of the human MAP1LC3 family and detection of a novel type of modification for MAP1LC3B." in: The Journal of biological chemistry, Vol. 278, Issue 31, pp. 29278-87, 2003 (PubMed).

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