Microtubule-Associated Protein 1 Light Chain 3 beta (MAP1LC3B) antibody

Antigen: Microtubule-Associated Protein 1 Light Chain 3 beta (MAP1LC3B)

Synonyms: LC3B, MAP1A/1BLC3, Mpl3, zbs559, Map1lc3, MGC93422, LC3b, MGC118046, 1010001C15Rik, zgc:56434, wu:fb60g11, map1lc3b, MGC76283, MAP1LC3B

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human, Chicken

Application: Immunohistochemistry (IHC), Western Blotting (WB)

Catalog no.: ABIN350466

Additional Information

Alternative name: microtubule-associated protein 1 light chain 3 beta (MAP1A/MAP1B LC3 B, Map1lc3b)

Immunogen: A synthetic peptide as a part of human, chicken microtubule-associated proteins 1A/1B light chain 3B (MAP1A/MAP1B LC3 B, Map1lc3b) conjugated to an immunogenic carrier protein has been used as the immunogen.

Format: Lyophilized

Description: Autophagy is the process by which endogenous proteins and damaged organelles are destroyed intracellularly.autophagy is postulated to be essential for cell homeostasis and cell remodeling during differentiation, metamorphosis, non-apoptotic cell death, and aging. Reduced levels ofautophagy have been described in some malignant tumors, and a role forautophagy in controlling the unregulated cell growth linked to cancer has been proposed. The product of this gene is a subunit of neuronal microtubule-associated MAP1A and MAP1B proteins, which are involved in microtubule assembly and important for neurogenesis. Studies on the rat homolog implicate a role for this gene inautophagy, a process that involves the bulk degradation of cytoplasmic component. FUNCTION: Probably involved in formation of autophagosomal vacuoles (autophagosomes). May mediate MAPlA and MAPlB microtubule binding activity during development of the nervous system SUBUNIT: 3 different light chains, LC1, LC2 and LC3, can associate with MAP1A and MAP1B proteins. SUBCELLULAR LOCATION: Cytoplasm. Intracytoplasmic membrane; Lipid-anchor. Cytoplasmic vesicle, autophagosome membrane; Lipid-anchor.Note: LC3-II binds to the autophagic membranes. Also known as: MLP3B, Map1alc3, Map1lc3, Microtubule-associated protein 1 light chain 3 beta, MAP1A/MAP1B LC3 B, MAP1A/1B light chain 3 B, MAP1 light chain 3-like protein 2,autophagy-related protein LC3 B,autophagy-related ubiquitin-like modifier LC3 B, Mpl3, MGC93422.

Specificity: Appears to be specific for MAP1LC3B.

Application Details

Application Notes: IHC, WB. Use at a dilution of 1 : 300 to 1 : 2000. The optimal dilution should be determined by the end user.

Purity: whole serum

Storage: Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.

Research Area: Autophagy

Restrictions: For Research Use only

Publications

Publications: He, Dang, Dai et al.: "Post-translational modifications of three members of the human MAP1LC3 family and detection of a novel type of modification for MAP1LC3B." in: The Journal of biological chemistry, Vol. 278, Issue 31, pp. 29278-87, 2003 (PubMed).

Tanida, Ueno, Kominami: "Human light chain 3/MAP1LC3B is cleaved at its carboxyl-terminal Met121 to expose Gly120 for lipidation and targeting to autophagosomal membranes." in: The Journal of biological chemistry, Vol. 279, Issue 46, pp. 47704-10, 2004 (PubMed).

Wiemann, Arlt, Huber et al.: "From ORFeome to biology: a functional genomics pipeline." in: Genome research, Vol. 14, Issue 10B, pp. 2136-44, 2004 (PubMed).