Mannose-6-Phosphate Receptor (Cation Dependent) (M6PR) (internal) antibody

Antigen: Mannose-6-Phosphate Receptor (Cation Dependent) (M6PR)

Synonyms: SMPR, MPR46, CD-MPR, FLJ32994, CD222, CI-MPR, Mpr300, AI661837, M6P/IGF2R, M6PR, CDMPR, MGC140730, MGC94300, m6pr, MGC68896, MGC130765, zgc:77757, wu:fj81h11, MGC89423, mprd

Epitope: internal

Clonality: Polyclonal

Host: Rabbit

Application: Immunohistochemistry (IHC), Western Blotting (WB)

Catalog no.: ABIN350471

Additional Information

Alternative name: MPRD (Cation dependent mannose-6-phosphate receptor) (internal region)

Swiss-Prot: P24668

Immunogen: A synthetic peptide from the internal region of mouse MPRD (Cation dependent mannose-6-phosphate receptor) conjugated to an immunogenic carrier protein was used as the immunogen. The antigen is homologous in rat.

Format: Lyophilized

Description: FUNCTION: Transport of phosphorylated lysosomal enzymes from the Golgi complex and the cell surface to lysosomes. Lysosomal enzymes bearing phosphomannosyl residues bind specifically to mannose-6-phosphate receptors in the Golgi apparatus and the resulting receptor-ligand complex is transported to an acidic prelyosomal compartment where the low pH mediates the dissociation of the complex. SUBUNIT: Homodimer. Binds GGA1, GGA2 and GGA3. SUBCELLULAR LOCATION: Lysosome membrane; Single-pass type I membrane protein. Also known as: CD Man-6-P receptor, CD-MPR, 46 kDa mannose 6-phosphate receptor, MPR 46, Cation-dependent mannose-6-phosphate receptor, M6pr, 46mpr.

Specificity: Appears to be specific for MPRD.

Application Details

Application Notes: IHC, WB. A dilution of 1 : 300 to 1 : 2000 is recommended. The optimal dilution should be determined by the end user. Not yet tested in other applications.

Purity: whole serum

Storage: Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.

Restrictions: For Research Use only

Images

WB on rat brain lysate using Rabbit antibody to internal region of MPRD (Cation dependent mannose-6-phosphate receptor): whole serum (ABIN350471) at 1 : 1000 dilution. Pre-absorption of the antibody with the immunising peptide completely abolishes the detected band.

Publications

Publications: Ludwig, Ruether, Metzger et al.: "Gene and pseudogene of the mouse cation-dependent mannose 6-phosphate receptor. Genomic organization, expression, and chromosomal localization." in: The Journal of biological chemistry, Vol. 267, Issue 17, pp. 12211-9, 1992 (PubMed).

Ma, Grubb, Sly: "Cloning, sequencing, and functional characterization of the murine 46-kDa mannose 6-phosphate receptor." in: The Journal of biological chemistry, Vol. 266, Issue 16, pp. 10589-95, 1991 (PubMed).

Koester, Nagel, von Figura et al.: "Molecular cloning of the mouse 46-kDa mannose 6-phosphate receptor (MPR 46)." in: Biological chemistry Hoppe-Seyler, Vol. 372, Issue 4, pp. 297-300, 1991 (PubMed).

Szebenyi, Rotwein: "Differential regulation of mannose 6-phosphate receptors and their ligands during the myogenic development of C2 cells." in: The Journal of biological chemistry, Vol. 266, Issue 9, pp. 5534-9, 1991 (PubMed).