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MAP-2 (AA 350-400) antibody

Details for Product No. ABIN350475
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Antigen
Synonyms p67, p67eIF2, MAP2A, MAP2B, MAP2C, MAP-2, Mtap-2, Mtap2, MAP2R, xmap2, MGC53131, a730034c02
Epitope
AA 350-400
(13), (12), (12), (12), (12), (5), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity
Rat (Rattus), Mouse (Murine)
(161), (98), (97), (68), (36), (24), (19), (4), (3), (3), (3), (1)
Host
Rabbit
(107), (87), (18), (3)
Clonality
Polyclonal
Conjugate
Un-conjugated
(9), (7), (7), (4), (4), (4), (4), (3), (3), (3), (3), (3), (3), (3), (1), (1), (1)
Application
Immunohistochemistry (IHC), Western Blotting (WB)
(157), (68), (54), (53), (49), (45), (30), (30), (26), (12), (3), (2), (2), (1)
Pubmed 6 references available
Quantity 100 µL
Options
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Catalog No. ABIN350475
454.67 $
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Immunogen A synthetic peptide from aa region 350-400 of mouse MAP2 conjugated to blue carrier protein was used as the antigen. The peptide is homologous in rat.
Specificity Specific for MAP2.
Purification Whole serum
Alternative Name MAP2
Background Regulates and organizes microtubules, may serve as a scaffolding protein for neuromodulatory activities.
Function: The exact function of MAP2 is unknown but MAPs may stabilize the microtubules against depolymerization. They also seem to have a stiffening effect on microtubules.
Subcellular location: Cytoplasm, cytoskeleton.
Developmental stage: Isoform MAP2C is expressed during embryonic brain development and until postanatal day 10. Isoform MAP2B is expressed throughout brain development. Also known as: Microtubule-associated protein 2, MAP 2, MAP-2, MAP2R.
Research Area Stem Cells, Cytoskeleton
Application Notes A dilution of 1 : 300 to 1 : 2000 is recommended.
The optimal dilution should be determined by the end user.
Restrictions For Research Use only
Format Lyophilized
Reconstitution Reconstitute in 150 µL of sterile water. Centrifuge to remove any insoluble material.
Handling Advice Avoid freeze and thaw cycles.
Storage 4 °C/-20 °C
Storage Comment Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.
Expiry Date 12 months
Supplier Images
anti-MAP-2 (AA 350-400) antibody IHC on rat brain (cortex) using Rabbit antibody to rat, mouse Mtap2 (Microtubule-associated protein 2, MAP-2, MAP 2, MAP2, MAP2R): whole serum (ABIN350475) at 1 : 1000 dilution. Pre-absorption of the antibody with the immunising peptide completely abolishes the immunostaining (not shown).
anti-MAP-2 (AA 350-400) antibody (2) IHC on rat brain (optical tract) using Rabbit antibody to rat, mouse Mtap2 (Microtubule-associated protein 2, MAP-2, MAP 2, MAP2, MAP2R): whole serum (ABIN350475) at 1 : 1000 dilution. Pre-absorption of the antibody with the immunising peptide completely abolishes the immunostaining (not shown).
General Kindler, Schulz, Goedert et al.: "Molecular structure of microtubule-associated protein 2b and 2c from rat brain." in: The Journal of biological chemistry, Vol. 265, Issue 32, pp. 19679-84, 1991 (PubMed).

Doll, Papandrikopoulou, Matus: "Nucleotide and amino acid sequences of embryonic rat MAP2c." in: Nucleic acids research, Vol. 18, Issue 2, pp. 361, 1990 (PubMed).

Kindler, Schwanke, Schulz et al.: "Complete cDNA sequence encoding rat high and low molecular weight MAP2." in: Nucleic acids research, Vol. 18, Issue 9, pp. 2822, 1990 (PubMed).

Papandrikopoulou, Doll, Tucker et al.: "Embryonic MAP2 lacks the cross-linking sidearm sequences and dendritic targeting signal of adult MAP2." in: Nature, Vol. 340, Issue 6235, pp. 650-2, 1989 (PubMed).

Ozer, Halpain: "Phosphorylation-dependent localization of microtubule-associated protein MAP2c to the actin cytoskeleton." in: Molecular biology of the cell, Vol. 11, Issue 10, pp. 3573-87, 2000 (PubMed).

Hoffert, Pisitkun, Wang et al.: "Quantitative phosphoproteomics of vasopressin-sensitive renal cells: regulation of aquaporin-2 phosphorylation at two sites." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 103, Issue 18, pp. 7159-64, 2006 (PubMed).

Validation Images
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