Microtubule Associated Protein 2 (MAP2) antibody

Antigen: Microtubule Associated Protein 2 (MAP2)

Synonyms: MAP2A, MAP2B, MAP2C, DKFZp686I2148, MAP2, MAP-2, Mtap-2, xmap2, MGC53131, a730034c02, map2, MGC145864

Clonality: Polyclonal

Host: Rabbit

Reactivity: Rat (Rattus), Mouse (Murine)

Conjugate: Un-conjugated

Application: Immunohistochemistry (IHC), Western Blotting (WB)

Catalog no.: ABIN350476

Additional Information

Alternative name: Mtap2 (Microtubule-associated protein 2, MAP-2, MAP2R)

UniProt: P15146

Immunogen: A synthetic peptide as a part of rat, mouse Mtap2 (Microtubule-associated protein 2, MAP-2, MAP 2, MAP2, MAP2R) conjugated to an immunogenic carrier protein was used as the immunogen.

Format: Lyophilized

Isotype: IgG

Description: Regulates and organizes microtubules; may serve as a scaffolding protein for neuromodulatory activities. FUNCTION: The exact function of MAP2 is unknown but MAPs may stabilize the microtubules against depolymerization. They also seem to have a stiffening effect on microtubules. SUBCELLULAR LOCATION: Cytoplasm, cytoskeleton. DEVELOPMENTAL STAGE: Isoform MAP2C is expressed during embryonic brain development and until postanatal day 10. Isoform MAP2B is expressed throughout brain development. Also known as: Microtubule-associated protein 2, MAP 2, MAP-2, MAP2R.

Specificity: Appears to be specific for MAP-2.

Application Details

Application Notes: IHC, WB. A working concentration of 10-50 µg/ml is recommended. The optimal concentration should be determined by the end user. Not yet tested in other applications.

Purity: IgG

Purification: The antibody is affinity-purified from rabbit antiserum by affinity chromatography using epitope-specific immunogen.

Storage: Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.

Restrictions: For Research Use only

Images

IF (confocal) on rat brain at a concentration of 30 µg/ml using Rabbit antibody to rat, mouse Mtap2 (Microtubule-associated protein 2, MAP-2, MAP 2, MAP2, MAP2R): IgG (ABIN350476), DAPI counterstained.

IF (confocal) on rat brain at a concentration of 30 µg/ml using Rabbit antibody to rat, mouse Mtap2 (Microtubule-associated protein 2, MAP-2, MAP 2, MAP2, MAP2R): IgG (ABIN350476), DAPI counterstained appearing in blue.

IF (confocal) on rat brain at a concentration of 30 µg/ml using Rabbit antibody to rat, mouse Mtap2 (Microtubule-associated protein 2, MAP-2, MAP 2, MAP2, MAP2R): IgG (ABIN350476), DAPI counterstained appearing in blue.

Publications

Kindler, Schulz, Goedert et al.: "Molecular structure of microtubule-associated protein 2b and 2c from rat brain." in: The Journal of biological chemistry, Vol. 265, Issue 32, pp. 19679-84, 1991 (PubMed).

Doll, Papandrikopoulou, Matus: "Nucleotide and amino acid sequences of embryonic rat MAP2c." in: Nucleic acids research, Vol. 18, Issue 2, pp. 361, 1990 (PubMed).

Kindler, Schwanke, Schulz et al.: "Complete cDNA sequence encoding rat high and low molecular weight MAP2." in: Nucleic acids research, Vol. 18, Issue 9, pp. 2822, 1990 (PubMed).

Papandrikopoulou, Doll, Tucker et al.: "Embryonic MAP2 lacks the cross-linking sidearm sequences and dendritic targeting signal of adult MAP2." in: Nature, Vol. 340, Issue 6235, pp. 650-2, 1989 (PubMed).

Ozer, Halpain: "Phosphorylation-dependent localization of microtubule-associated protein MAP2c to the actin cytoskeleton." in: Molecular biology of the cell, Vol. 11, Issue 10, pp. 3573-87, 2000 (PubMed).

Hoffert, Pisitkun, Wang et al.: "Quantitative phosphoproteomics of vasopressin-sensitive renal cells: regulation of aquaporin-2 phosphorylation at two sites." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 103, Issue 18, pp. 7159-64, 2006 (PubMed).