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MAP2 antibody (MAP-2)

Details for Product anti-MAP-2 Antibody No. ABIN350478, Supplier: Log in to see
Antigen
  • p67
  • p67eIF2
  • MAP2A
  • MAP2B
  • MAP2C
  • MAP-2
  • Mtap-2
  • Mtap2
  • MAP2R
  • xmap2
  • MGC53131
  • a730034c02
  • microtubule-associated protein 2
  • methionyl aminopeptidase 2
  • microtubule-associated protein 2-like
  • MAP2
  • METAP2
  • Map2
  • map2
  • LOC100339229
Reactivity
Rat (Rattus), Mouse (Murine)
318
209
170
122
31
19
16
14
11
9
4
1
1
1
1
1
Host
Sheep
178
170
19
3
Clonality
Polyclonal
Conjugate
This MAP2 antibody is un-conjugated
17
16
12
12
8
8
5
4
4
4
4
4
4
4
4
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
1
Application
Immunohistochemistry (IHC), Western Blotting (WB)
285
155
118
100
97
62
45
26
25
24
14
13
6
4
4
3
3
2
2
1
1
Options
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Immunogen A synthetic peptide from mouse Mtap2 (Microtubule-associated protein 2, MAP-2, MAP 2, MAP2, MAP2R) conjugated to an immunogenic carrier protein was used as the antigen.
Isotype IgG
Specificity Specific for MAP-2.
Alternative Name MAP 2
Background Regulates and organizes microtubules, may serve as a scaffolding protein for neuromodulatory activities.
Function: The exact function of MAP2 is unknown but MAPs may stabilize the microtubules against depolymerization. They also seem to have a stiffening effect on microtubules.
Subcellular location: Cytoplasm, cytoskeleton.
Developmental stage: Isoform MAP2C is expressed during embryonic brain development and until postanatal day 10. Isoform MAP2B is expressed throughout brain development. Also known as: Microtubule-associated protein 2, MAP 2, MAP-2, MAP2R.
Application Notes A concentration of 10-50 µg/ml is recommended.
The optimal concentration should be determined by the end user.
Not yet tested in other applications.
Restrictions For Research Use only
Format Lyophilized
Reconstitution Reconstitute in 100 µL of sterile water. Centrifuge to remove any insoluble material.
Handling Advice Avoid freeze and thaw cycles.
Storage 4 °C/-20 °C
Storage Comment Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.
Expiry Date 12 months
Background publications Hoffert, Pisitkun, Wang, Shen, Knepper: "Quantitative phosphoproteomics of vasopressin-sensitive renal cells: regulation of aquaporin-2 phosphorylation at two sites." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 103, Issue 18, pp. 7159-64, 2006 (PubMed).

Ozer, Halpain: "Phosphorylation-dependent localization of microtubule-associated protein MAP2c to the actin cytoskeleton." in: Molecular biology of the cell, Vol. 11, Issue 10, pp. 3573-87, 2000 (PubMed).

Kindler, Schulz, Goedert, Garner: "Molecular structure of microtubule-associated protein 2b and 2c from rat brain." in: The Journal of biological chemistry, Vol. 265, Issue 32, pp. 19679-84, 1991 (PubMed).

Doll, Papandrikopoulou, Matus: "Nucleotide and amino acid sequences of embryonic rat MAP2c." in: Nucleic acids research, Vol. 18, Issue 2, pp. 361, 1990 (PubMed).

Kindler, Schwanke, Schulz, Garner: "Complete cDNA sequence encoding rat high and low molecular weight MAP2." in: Nucleic acids research, Vol. 18, Issue 9, pp. 2822, 1990 (PubMed).

Papandrikopoulou, Doll, Tucker, Garner, Matus: "Embryonic MAP2 lacks the cross-linking sidearm sequences and dendritic targeting signal of adult MAP2." in: Nature, Vol. 340, Issue 6235, pp. 650-2, 1989 (PubMed).