Mucolipin 1 (Mcoln1, MCLN1,TRPML1) (N-Term) antibody

Antigen: Mucolipin 1 (Mcoln1, MCLN1,TRPML1)

Binding Site: N-Term

Clonality: Polyclonal

Host: Rabbit

Reactivity: Mouse (Murine)

Application: Immunohistochemistry (IHC), Western Blotting (WB)

Catalog no.: ABIN350481

Additional Information

UniProt: Q99J21

Immunogen: A synthetic peptide from the n-terminal part of mouse Mucolipin 1 conjugated to an immunogenic carrier protein was used as the immunogen.

Format: Lyophilized

Description: Polycystin cation channel contains the cation channel region of PKD1 and PKD2 proteins; pfam08016 FUNCTION: Cationic channel probably playing a role in the endocytic pathway and in the control of membrane trafficking of proteins and lipids. Could play a major role in Ca(2+) transport regulating lysosomal exocytosis. SUBUNIT: Forms multimeric complexes. SUBCELLULAR LOCATION: Cell membrane; Multi-pass membrane protein. Late endosome membrane; Multi-pass membrane protein. Lysosome membrane; Multi-pass membrane protein. TISSUE SPECIFICITY: Widely expressed, with the highest expression in brain, liver and kidney. MISCELLANEOUS: Channel function is transiently modulated by changes in Ca(2+), and inhibited by a reduction of pH; pH changes modify the aggregation state of unitary channels. SIMILARITY: Belongs to the transient receptor family, polycystin subfamily. Also known as:Mucolipin-1, Mucolipidin, Mcoln1,Mucolipin 1, MCLN1, TRPML1, MGC7172, 2210015I05Rik.

Specificity: Appears to be specific for Mucolipin 1.

Application Details

Application Notes: IHC, WB. A dilution of 1: 300 to 1: 3000 is recommended. The optimal dilution should be determined by the end user.

Purity: whole serum

Purification: The antibody is affinity-purified from rabbit antiserum by affinity chromatography using epitope-specific immunogen.

Storage: Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.

Restrictions: For Research Use only

Publications

Falardeau, Kennedy, Acierno et al.: "Cloning and characterization of the mouse Mcoln1 gene reveals an alternatively spliced transcript not seen in humans." in: BMC genomics, Vol. 3, Issue 1, pp. 3, 2003 (PubMed).

Ballif, Villén, Beausoleil et al.: "Phosphoproteomic analysis of the developing mouse brain." in: Molecular & cellular proteomics : MCP, Vol. 3, Issue 11, pp. 1093-101, 2004 (PubMed).

Carninci, Kasukawa, Katayama et al.: "The transcriptional landscape of the mammalian genome." in: Science (New York, N.Y.), Vol. 309, Issue 5740, pp. 1559-63, 2005 (PubMed).

Munton, Tweedie-Cullen, Livingstone-Zatchej et al.: "Qualitative and quantitative analyses of protein phosphorylation in naive and stimulated mouse synaptosomal preparations." in: Molecular & cellular proteomics : MCP, Vol. 6, Issue 2, pp. 283-93, 2007 (PubMed).

Lee, Xu, Chen et al.: "Mitochondrial phosphoproteome revealed by an improved IMAC method and MS/MS/MS." in: Molecular & cellular proteomics : MCP, Vol. 6, Issue 4, pp. 669-76, 2007 (PubMed).