Myelin Basic Protein (MBP) (N-Term) antibody

Antigen: Myelin Basic Protein (MBP)

Synonyms: MGC99675, mld, shi, Hmbpr, C76307, R75289, golli-mbp, Mbps, cb274, fj33b11, MGC136630, wu:fj33b11, wu:fq15b02, zgc:136630, Mbp, MBP, MGC115135, DKFZp459G2410, DKFZp459P2220

Epitope: N-Term

Clonality: Polyclonal

Host: Rabbit

Application: Immunohistochemistry (IHC), Western Blotting (WB)

Catalog no.: ABIN350504

Additional Information

Alternative name: Myelin basic protein (MBP) (n-terminal region)

Swiss-Prot: P02686

Immunogen: A synthetic peptide to n-terminal region of human Myelin basic protein (MBP) conjugated to an immunogenic carrier protein was used as the immunogen.

Format: Lyophilized

Description: FUNCTION: The classic group of MBP isoforms (isoform 4-isoform 14) are with PLP the most abundant protein components of the myelin membrane in the CNS. They have a role in both its formation and stabilization. The smaller isoforms might have an important role in remyelination of denuded axons in multiple sclerosis. The non-classic group of MBP isoforms (isoform 1-isoform 3/Golli-MBPs) may preferentially have a role in the early developing brain long before myelination, maybe as components of transcriptional complexes, and may also be involved in signaling pathways in T-cells and neural cells. Differential splicing events combined with optional post-translational modifications give a wide spectrum of isomers, with each of them potentially having a specialized function. Induces T-cell proliferation. The reduction in the surface charge of citrullinated and/or methylated MBP could result in a weakened attachment to the myelin membrane. This mechanism could be operative in demyelinating diseases such as chronical multiple sclerosis (MS), and fulminating MS (Marburg disease). SUBCELLULAR LOCATION: Myelin membrane; Peripheral membrane protein; Cytoplasmic side. Note: Cytoplasmic side of myelin. TISSUE SPECIFICITY: MBP isoforms are found in both the central and the peripheral nervous system, whereas Golli-MBP isoforms are expressed in fetal thymus, spleen and spinal cord, as well as in cell lines derived from the immune system. Also known as: MBP, Myelin A1 protein, Myelin membrane encephalitogenic protein.

Specificity: Appears to be specific for MBP.

Application Details

Application Notes: IHC, WB. A dilution of 1: 300 to 1: 2000 is recommended. The optimal dilution should be determined by the end user. Not yet tested in other applications.

Purity: whole serum

Storage: Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.

Restrictions: For Research Use only

Publications

Publications: Kamholz, de Ferra, Puckett et al.: "Identification of three forms of human myelin basic protein by cDNA cloning." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 83, Issue 13, pp. 4962-6, 1986 (PubMed).

Roth, Kronquist, Pretorius et al.: "Isolation and characterization of a cDNA coding for a novel human 17.3K myelin basic protein (MBP) variant." in: Journal of neuroscience research, Vol. 16, Issue 1, pp. 227-38, 1986 (PubMed).

Roth, Kronquist, Kerlero de Rosbo et al.: "Evidence for the expression of four myelin basic protein variants in the developing human spinal cord through cDNA cloning." in: Journal of neuroscience research, Vol. 17, Issue 4, pp. 321-8, 1987 (PubMed).

Pribyl, Campagnoni, Kampf et al.: "The human myelin basic protein gene is included within a 179-kilobase transcription unit: expression in the immune and central nervous systems." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 90, Issue 22, pp. 10695-9, 1993 (PubMed).

Li, Huang, Lue et al.: "Structure of a human autoimmune TCR bound to a myelin basic protein self-peptide and a multiple sclerosis-associated MHC class II molecule." in: The EMBO journal, Vol. 24, Issue 17, pp. 2968-79, 2005 (PubMed).