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ATP1A1 antibody (ATPase, Na+/K+ Transporting, alpha 1 Polypeptide)

Details for Product anti-ATP1A1 Antibody No. ABIN350506, Supplier: Login to see
Antigen
  • Nkaa1b
  • K-ATPase
  • Na+K+ATPase
  • atp1a1
  • atp1a1a.1
  • Atpa-1
  • BC010319
Reactivity
Mouse (Murine), Rat (Rattus), Human
90
89
73
9
7
7
7
6
1
1
1
Host
Rabbit
106
13
1
Clonality
Polyclonal
Conjugate
This ATP1A1 antibody is un-conjugated
5
3
3
3
3
3
3
3
3
3
3
3
Application
Immunohistochemistry (IHC), Western Blotting (WB)
78
37
31
19
16
15
7
6
2
1
1
Supplier
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Immunogen A synthetic peptide from mouse Na+/K+ -ATPase alpha 1 subunit (Sodium/potassium-transporting ATPase alpha-1) conjugated to an immunogenic carrier protein was used as the antigen.
Specificity Specific for Na+/K+ -ATPase alpha 1 subunit.
Purification Whole serum
Alternative Name ATP1A1 (ATP1A1 Antibody Abstract)
Background Function: This is the catalytic component of the active enzyme, which catalyzes the hydrolysis of ATP coupled with the exchange of sodium and potassium ions across the plasma membrane. This action creates the electrochemical gradient of sodium and potassium ions, providing the energy for active transport of various nutrients. Melanosome.
Catalytic activity: ATP + H2O + Na+(In) + K+(Out) = ADP + phosphate + Na+(Out) + K+(In).
Subunit: Composed of three subunits: alpha (catalytic), beta and gamma.
Subcellular location: Membrane, Multi-pass membrane protein. Also known as: AT1A1, Sodium/potassium-transporting ATPase subunit alpha-1, Sodium pump subunit alpha-1, Na(+)/K(+) ATPase alpha-1 subunit, Atp1a1.
Pathways
Application Notes A dilution of 1 : 300 to 1 : 2000 is recommended.
The optimal dilution should be determined by the end user.
Not yet tested in other applications.
Restrictions For Research Use only
Format Lyophilized
Reconstitution Reconstitute in 100 µL of sterile water. Centrifuge to remove any insoluble material.
Handling Advice Avoid freeze and thaw cycles.
Storage 4 °C/-20 °C
Storage Comment Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.
Expiry Date 12 months
Background publications Ballif, Carey, Sunyaev et al.: "Large-scale identification and evolution indexing of tyrosine phosphorylation sites from murine brain." in: Journal of proteome research, Vol. 7, Issue 1, pp. 311-8, 2008 (PubMed).

Wolf-Yadlin, Hautaniemi, Lauffenburger et al.: "Multiple reaction monitoring for robust quantitative proteomic analysis of cellular signaling networks." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 104, Issue 14, pp. 5860-5, 2007 (PubMed).

Munton, Tweedie-Cullen, Livingstone-Zatchej et al.: "Qualitative and quantitative analyses of protein phosphorylation in naive and stimulated mouse synaptosomal preparations." in: Molecular & cellular proteomics : MCP, Vol. 6, Issue 2, pp. 283-93, 2007 (PubMed).

Vosseller, Hansen, Chalkley et al.: "Quantitative analysis of both protein expression and serine / threonine post-translational modifications through stable isotope labeling with dithiothreitol." in: Proteomics, Vol. 5, Issue 2, pp. 388-98, 2005 (PubMed).