Na+/K+ -ATPasealpha 1 Subunit (Sodium/potassium-Transporting ATPasealpha-1) antibody

Antigen: Na+/K+ -ATPasealpha 1 Subunit (Sodium/potassium-Transporting ATPasealpha-1)

Clonality: Polyclonal

Host: Rabbit

Application: Immunohistochemistry (IHC), Western Blotting (WB)

Catalog no.: ABIN350506

Additional Information

UniProt: Q8VDN2

Immunogen: A synthetic peptide from the n-terminal part of mouse Na+/K+ -ATPase alpha 1 subunit (Sodium/potassium-transporting ATPase alpha-1) conjugated to an immunogenic carrier protein was used as the immunogen. The antigen is homologous in rat.

Format: Lyophilized

Description: FUNCTION: This is the catalytic component of the active enzyme, which catalyzes the hydrolysis of ATP coupled with the exchange of sodium and potassium ions across the plasma membrane. This action creates the electrochemical gradient of sodium and potassium ions, providing the energy for active transport of various nutrients. Melanosome. CATALYTIC ACTIVITY: ATP + H2O + Na+(In) + K+(Out) = ADP + phosphate + Na+(Out) + K+(In). SUBUNIT: Composed of three subunits: alpha (catalytic), beta and gamma. SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein. Also known as: AT1A1, Sodium/potassium-transporting ATPase subunit alpha-1, Sodium pump subunit alpha-1, Na(+)/K(+) ATPase alpha-1 subunit, Atp1a1.

Specificity: Appears to be specific for Na+/K+ -ATPase alpha 1 subunit.

Application Details

Application Notes: IHC, WB. A dilution of 1 : 300 to 1 : 2000 is recommended. The optimal dilution should be determined by the end user. Not yet tested in other applications.

Purity: whole serum

Purification: The antibody is affinity-purified from rabbit antiserum by affinity chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide is removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

Storage: Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.

Restrictions: For Research Use only

Publications

Vosseller, Hansen, Chalkley et al.: "Quantitative analysis of both protein expression and serine / threonine post-translational modifications through stable isotope labeling with dithiothreitol." in: Proteomics, Vol. 5, Issue 2, pp. 388-98, 2005 (PubMed).

Munton, Tweedie-Cullen, Livingstone-Zatchej et al.: "Qualitative and quantitative analyses of protein phosphorylation in naive and stimulated mouse synaptosomal preparations." in: Molecular & cellular proteomics : MCP, Vol. 6, Issue 2, pp. 283-93, 2007 (PubMed).

Wolf-Yadlin, Hautaniemi, Lauffenburger et al.: "Multiple reaction monitoring for robust quantitative proteomic analysis of cellular signaling networks." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 104, Issue 14, pp. 5860-5, 2007 (PubMed).

Ballif, Carey, Sunyaev et al.: "Large-scale identification and evolution indexing of tyrosine phosphorylation sites from murine brain." in: Journal of proteome research, Vol. 7, Issue 1, pp. 311-8, 2008 (PubMed).