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Details for Product No. ABIN350506

Na+/K+ -ATPasealpha 1 Subunit (Sodium/potassium-Transporting ATPasealpha-1) antibody

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Antigen
Reactivity
Mouse (Murine), Rat (Rattus)
Host
Rabbit
Clonality Polyclonal
Application
Immunohistochemistry (IHC), Western Blotting (WB)
Pubmed 4 references available
Catalog no. ABIN350506
Quantity 100 µL
Price
454.67 $   Plus shipping costs $45.00
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Availability Will be delivered in 7 to 8 Business Days
Immunogen A synthetic peptide from the n-terminal part of mouse Na+/K+ -ATPase alpha 1 subunit (Sodium/potassium-transporting ATPase alpha-1) conjugated to an immunogenic carrier protein was used as the immunogen. The antigen is homologous in rat.
Specificity Appears to be specific for Na+/K+ -ATPase alpha 1 subunit.
Purification The antibody is affinity-purified from rabbit antiserum by affinity chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide is removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Purity whole serum
Background Function: This is the catalytic component of the active enzyme, which catalyzes the hydrolysis of ATP coupled with the exchange of sodium and potassium ions across the plasma membrane. This action creates the electrochemical gradient of sodium and potassium ions, providing the energy for active transport of various nutrients. Melanosome.
Catalytic activity: ATP + H2O + Na+(In) + K+(Out) = ADP + phosphate + Na+(Out) + K+(In).
Subunit: Composed of three subunits: alpha (catalytic), beta and gamma.
Subcellular location: Membrane, Multi-pass membrane protein. Also known as: AT1A1, Sodium/potassium-transporting ATPase subunit alpha-1, Sodium pump subunit alpha-1, Na(+)/K(+) ATPase alpha-1 subunit, Atp1a1.
UniProt Q8VDN2
Application Notes IHC, WB. A dilution of 1 : 300 to 1 : 2000 is recommended. The optimal dilution should be determined by the end user. Not yet tested in other applications.
Restrictions For Research Use only
Format Lyophilized
Storage -20 °C
Vosseller, Hansen, Chalkley et al.: "Quantitative analysis of both protein expression and serine / threonine post-translational modifications through stable isotope labeling with dithiothreitol." in: Proteomics, Vol. 5, Issue 2, pp. 388-98, 2005 (PubMed).

Munton, Tweedie-Cullen, Livingstone-Zatchej et al.: "Qualitative and quantitative analyses of protein phosphorylation in naive and stimulated mouse synaptosomal preparations." in: Molecular & cellular proteomics : MCP, Vol. 6, Issue 2, pp. 283-93, 2007 (PubMed).

Wolf-Yadlin, Hautaniemi, Lauffenburger et al.: "Multiple reaction monitoring for robust quantitative proteomic analysis of cellular signaling networks." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 104, Issue 14, pp. 5860-5, 2007 (PubMed).

Ballif, Carey, Sunyaev et al.: "Large-scale identification and evolution indexing of tyrosine phosphorylation sites from murine brain." in: Journal of proteome research, Vol. 7, Issue 1, pp. 311-8, 2008 (PubMed).

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