Neural Precursor Cell Expressed, Developmentally Down-Regulated 4 (NEDD4) antibody

Antigen: Neural Precursor Cell Expressed, Developmentally Down-Regulated 4 (NEDD4)

Synonyms: RPF1, NEDD4-1, KIAA0093, MGC176705, Nedd4a, Nedd4-1, AA959633, AL023035, AU019897, mKIAA0093, E430025J12Rik, CG32184, CG7555, DNedd4, dNedd4-1, nedd4, DmelCG42279, CG42279, GB16853, NEDD4, Nedd4

Clonality: Polyclonal

Host: Rabbit

Application: Immunohistochemistry (IHC), Western Blotting (WB)

Catalog no.: ABIN350509

Additional Information

Alternative name: NEDD4 (NEDD-4, E3 ubiquitin-protein ligase NEDD4) (WW1 domain)

Swiss-Prot: P46935

Immunogen: A synthetic peptide from the WW1 domain of mouse NEDD4 (NEDD-4, E3 ubiquitin-protein ligase NEDD4) conjugated to an immunogenic carrier protein was used as the immunogen. The antigen shares 96% identity with human NEDD4.

Format: Lyophilized

Description: FUNCTION: E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. FUNCTION: Involved in the embryonic development and differentiation of the central nervous system. SUBCELLULAR LOCATION: Cytoplasm. TISSUE SPECIFICITY: Brain. Also known as: Neural precursor cell expressed developmentally down-regulated protein 4, E3 ubiquitin-protein ligase NEDD4, NEDD-4.

Specificity: Appears to be specific for NEDD4.

Application Details

Application Notes: IHC, WB. A dilution of 1: 300 to 1 : 2000 is recommended. The optimal dilution should be determined by the end user. Not yet tested in other applications.

Purity: whole serum

Storage: Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.

Restrictions: For Research Use only

Publications

Publications: Kumar, Tomooka, Noda: "Identification of a set of genes with developmentally down-regulated expression in the mouse brain." in: Biochemical and biophysical research communications, Vol. 185, Issue 3, pp. 1155-61, 1992 (PubMed).

Hatakeyama, Jensen, Weissman: "Subcellular localization and ubiquitin-conjugating enzyme (E2) interactions of mammalian HECT family ubiquitin protein ligases." in: The Journal of biological chemistry, Vol. 272, Issue 24, pp. 15085-92, 1997 (PubMed).

Ermekova, Zambrano, Linn et al.: "The WW domain of neural protein FE65 interacts with proline-rich motifs in Mena, the mammalian homolog of Drosophila enabled." in: The Journal of biological chemistry, Vol. 272, Issue 52, pp. 32869-77, 1998 (PubMed).

Carninci, Kasukawa, Katayama et al.: "The transcriptional landscape of the mammalian genome." in: Science (New York, N.Y.), Vol. 309, Issue 5740, pp. 1559-63, 2005 (PubMed).

Trinidad, Specht, Thalhammer et al.: "Comprehensive identification of phosphorylation sites in postsynaptic density preparations." in: Molecular & cellular proteomics : MCP, Vol. 5, Issue 5, pp. 914-22, 2006 (PubMed).