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Details for Product No. ABIN350510

NEDD4 WW Domain Binding Protein 5 (NFIP1) (N-Term), (Cytoplasmic Domain) antibody

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N-Term, Cytoplasmic Domain
Mouse (Murine), Rat (Rattus), Human
Clonality Polyclonal
Immunohistochemistry (IHC), Western Blotting (WB)
Pubmed 3 references available
Catalog no. ABIN350510
Quantity 100 µL
454.67 $   Plus shipping costs $45.00
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Availability Will be delivered in 7 to 8 Business Days
Immunogen A synthetic peptide from the n-terminal (cytoplasmic) domain of mouse NEDD4 WW domain binding protein 5 (Ndfip1, NFIP1) conjugated to an immunogenic carrier protein was used as the immunogen. The antigen shares 96% identity with human and rat Ndfip1.
Specificity Appears to be specific for Ndfip1.
Purification The antibody is affinity-purified from rabbit antiserum by affinity chromatography using epitope-specific immunogen.
Purity whole serum
Background Function: May play a role in Golgi structure maintenance.
Subunit: Interacts with NEDD4. Interacts in vitro with NEDD4L, SR140, WWP2 and ITCH.
Subcellular location: Golgi apparatus membrane, Multi-pass membrane protein.
Tissue specificity: Widely expressed. Also known as: NEDD4 family-interacting protein 1, Ndfip1, N4wbp5, NFIP1.
UniProt Q8R0W6
Application Notes IHC, WB. A dilution of 1: 500 to 1 : 2000 is recommended. The optimal dilution should be determined by the end user. Not yet tested in other applications.
Restrictions For Research Use only
Format Lyophilized
Storage -20 °C
Jolliffe, Harvey, Haines et al.: "Identification of multiple proteins expressed in murine embryos as binding partners for the WW domains of the ubiquitin-protein ligase Nedd4." in: The Biochemical journal, Vol. 351 Pt 3, pp. 557-65, 2001 (PubMed).

Harvey, Shearwin-Whyatt, Fotia et al.: "N4WBP5, a potential target for ubiquitination by the Nedd4 family of proteins, is a novel Golgi-associated protein." in: The Journal of biological chemistry, Vol. 277, Issue 11, pp. 9307-17, 2002 (PubMed).

Carninci, Kasukawa, Katayama et al.: "The transcriptional landscape of the mammalian genome." in: Science (New York, N.Y.), Vol. 309, Issue 5740, pp. 1559-63, 2005 (PubMed).