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NEDD4 WW domain binding protein 5 (NFIP1) (N-Term, Cytoplasmic Domain) antibody
N-Term, Cytoplasmic Domain
Immunohistochemistry (IHC), Western Blotting (WB)
|3 references available|
|Price||454.67 $ Plus shipping costs $45.00|
|Availability||Will be delivered in 7 to 8 Business Days|
|Immunogen||A synthetic peptide from the n-terminal (cytoplasmic) domain of mouse NEDD4 WW domain binding protein 5 (Ndfip1, NFIP1) conjugated to an immunogenic carrier protein was used as the immunogen. The antigen shares 96% identity with human and rat Ndfip1.|
|Description||FUNCTION: May play a role in Golgi structure maintenance. SUBUNIT: Interacts with NEDD4. Interacts in vitro with NEDD4L, SR140, WWP2 and ITCH. SUBCELLULAR LOCATION: Golgi apparatus membrane; Multi-pass membrane protein. TISSUE SPECIFICITY: Widely expressed. Also known as: NEDD4 family-interacting protein 1, Ndfip1, N4wbp5, NFIP1.|
|Specificity||Appears to be specific for Ndfip1.|
|Application Notes||IHC, WB. A dilution of 1: 500 to 1 : 2000 is recommended. The optimal dilution should be determined by the end user. Not yet tested in other applications.|
|Purification||The antibody is affinity-purified from rabbit antiserum by affinity chromatography using epitope-specific immunogen.|
|Storage||Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.|
|Restrictions||For Research Use only|
Jolliffe, Harvey, Haines et al.: "Identification of multiple proteins expressed in murine embryos as binding partners for the WW domains of the ubiquitin-protein ligase Nedd4." in: The Biochemical journal, Vol. 351 Pt 3, pp. 557-65, 2001 (PubMed).
Harvey, Shearwin-Whyatt, Fotia et al.: "N4WBP5, a potential target for ubiquitination by the Nedd4 family of proteins, is a novel Golgi-associated protein." in: The Journal of biological chemistry, Vol. 277, Issue 11, pp. 9307-17, 2002 (PubMed).
Carninci, Kasukawa, Katayama et al.: "The transcriptional landscape of the mammalian genome." in: Science (New York, N.Y.), Vol. 309, Issue 5740, pp. 1559-63, 2005 (PubMed).