NEDD4 WW domain binding protein 5 (NFIP1) (N-Term, Cytoplasmic Domain) antibody

Antigen: NEDD4 WW domain binding protein 5 (NFIP1)

Binding Site: N-Term, Cytoplasmic Domain

Clonality: Polyclonal

Host: Rabbit

Application: Immunohistochemistry (IHC), Western Blotting (WB)

Catalog no.: ABIN350510

Additional Information

UniProt: Q8R0W6

Immunogen: A synthetic peptide from the n-terminal (cytoplasmic) domain of mouse NEDD4 WW domain binding protein 5 (Ndfip1, NFIP1) conjugated to an immunogenic carrier protein was used as the immunogen. The antigen shares 96% identity with human and rat Ndfip1.

Format: Lyophilized

Description: FUNCTION: May play a role in Golgi structure maintenance. SUBUNIT: Interacts with NEDD4. Interacts in vitro with NEDD4L, SR140, WWP2 and ITCH. SUBCELLULAR LOCATION: Golgi apparatus membrane; Multi-pass membrane protein. TISSUE SPECIFICITY: Widely expressed. Also known as: NEDD4 family-interacting protein 1, Ndfip1, N4wbp5, NFIP1.

Specificity: Appears to be specific for Ndfip1.

Application Details

Application Notes: IHC, WB. A dilution of 1: 500 to 1 : 2000 is recommended. The optimal dilution should be determined by the end user. Not yet tested in other applications.

Purity: whole serum

Purification: The antibody is affinity-purified from rabbit antiserum by affinity chromatography using epitope-specific immunogen.

Storage: Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.

Restrictions: For Research Use only

Publications

Jolliffe, Harvey, Haines et al.: "Identification of multiple proteins expressed in murine embryos as binding partners for the WW domains of the ubiquitin-protein ligase Nedd4." in: The Biochemical journal, Vol. 351 Pt 3, pp. 557-65, 2001 (PubMed).

Harvey, Shearwin-Whyatt, Fotia et al.: "N4WBP5, a potential target for ubiquitination by the Nedd4 family of proteins, is a novel Golgi-associated protein." in: The Journal of biological chemistry, Vol. 277, Issue 11, pp. 9307-17, 2002 (PubMed).

Carninci, Kasukawa, Katayama et al.: "The transcriptional landscape of the mammalian genome." in: Science (New York, N.Y.), Vol. 309, Issue 5740, pp. 1559-63, 2005 (PubMed).