Nedd4 Family Interacting Protein 1 (NDFIP1) (N-Term) antibody

Details for Product No. ABIN350510
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Antigen
Synonyms zgc:55546, wu:fc17e11, n4wbp5, MGC108163, ndfip1, NDFIP1, N4WBP5, 0610010M22Rik, N4wbp5
Epitope
N-Term
(14), (8), (7), (2), (1), (1)
Reactivity
Mouse (Murine)
(24), (18), (5), (1), (1)
Host
Rabbit
(20), (7), (3), (2)
Clonality
Polyclonal
Conjugate
Un-conjugated
(1), (1), (1), (1), (1), (1)
Application
Immunohistochemistry (IHC), Western Blotting (WB)
(30), (26), (2), (1)
Pubmed 3 references available
Quantity 100 µL
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Catalog No. ABIN350510
454.67 $
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Immunogen A synthetic peptide from the n-terminal (cytoplasmic) domain of mouse NEDD4 WW domain binding protein 5 (Ndfip1, NFIP1) conjugated to an immunogenic carrier protein was used as the antigen. The antigen shares 96% identity with human and rat Ndfip1.
Specificity Specific for Ndfip1.
Purification Whole serum
Alternative Name NDFIP1
Background Function: May play a role in Golgi structure maintenance.
Subunit: Interacts with NEDD4. Interacts in vitro with NEDD4L, SR140, WWP2 and ITCH.
Subcellular location: Golgi apparatus membrane, Multi-pass membrane protein.
Tissue specificity: Widely expressed. Also known as: NEDD4 family-interacting protein 1, Ndfip1, N4wbp5, NFIP1.
Application Notes A dilution of 1: 500 to 1 : 2000 is recommended.
The optimal dilution should be determined by the end user.
Not yet tested in other applications.
Restrictions For Research Use only
Format Lyophilized
Reconstitution Reconstitute in 100 µL of sterile water. Centrifuge to remove any insoluble material.
Handling Advice Avoid freeze and thaw cycles.
Storage 4 °C/-20 °C
Storage Comment Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.
Expiry Date 12 months
General Jolliffe, Harvey, Haines et al.: "Identification of multiple proteins expressed in murine embryos as binding partners for the WW domains of the ubiquitin-protein ligase Nedd4." in: The Biochemical journal, Vol. 351 Pt 3, pp. 557-65, 2001 (PubMed).

Harvey, Shearwin-Whyatt, Fotia et al.: "N4WBP5, a potential target for ubiquitination by the Nedd4 family of proteins, is a novel Golgi-associated protein." in: The Journal of biological chemistry, Vol. 277, Issue 11, pp. 9307-17, 2002 (PubMed).

Carninci, Kasukawa, Katayama et al.: "The transcriptional landscape of the mammalian genome." in: Science (New York, N.Y.), Vol. 309, Issue 5740, pp. 1559-63, 2005 (PubMed).

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