Neural Precursor Cell Expressed, Developmentally Down-Regulated 8 (NEDD8) (N-Term) antibody

Antigen: Neural Precursor Cell Expressed, Developmentally Down-Regulated 8 (NEDD8)

Synonyms: Nedd-8, FLJ43224, MGC104393, MGC125896, MGC125897, Rub1, MGC105320, CG10679, DmelCG10679, nedd8, ubiquitin, si:zc14a17.5, NEDD8, GB17785, LOC100009008

Epitope: N-Term

Clonality: Polyclonal

Host: Rabbit

Application: Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)

Catalog no.: ABIN350512

Additional Information

Alternative name: NEDD8 (Ubiquitin-like protein Nedd8, Neddylin) (n-terminal)

Swiss-Prot: P29595

Immunogen: A synthetic peptide from the n-terminal of mouse NEDD8 (Ubiquitin-like protein Nedd8, Neddylin) conjugated to immunogenic carrier protein has been used as the immunogen. The antigen is homologous in human, rat, zebrafish, drosophila and xenopus.

Format: Lyophilized

Description: Function: Ubiquitin-like protein which plays an important role in cell cycle control and embryogenesis. Covalent attachment to its substrates requires prior activation by the E1 complex UBE1C-APPBP1 and linkage to the E2 enzyme UBE2M. Attachment of NEDD8 to cullins activates their associated E3 ubiquitin ligase activity, and thus promotes polyubiquitination and proteasomal degradation of cyclins and other regulatory protein. Subcellular location: Mainly nuclear Tissue specificity: Ubiquitously expressed (at protein level). Post-translational modification: Cleavage of precursor form by UCHL3 or SENP8 is necessary for function Also known as: Ubiquitin-like protein Nedd8, Neddylin, Neural precursor cell expressed developmentally down-regulated protein 8, NEDD-8.

Specificity: Appears to be specific for NEDD8.

Application Details

Application Notes: IHC (both on frozen and paraffin embedded tissues), WB. Use at a dilution of 1 : 300 to 1 : 2000. The optimal dilution should be determined by the end user. Not yet tested in other applications.

Purity: whole serum

Storage: Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.

Restrictions: For Research Use only

Publications

Publications: Kumar, Tomooka, Noda: "Identification of a set of genes with developmentally down-regulated expression in the mouse brain." in: Biochemical and biophysical research communications, Vol. 185, Issue 3, pp. 1155-61, 1992 (PubMed).

Kumar, Yoshida, Noda: "Cloning of a cDNA which encodes a novel ubiquitin-like protein." in: Biochemical and biophysical research communications, Vol. 195, Issue 1, pp. 393-9, 1993 (PubMed).

Hori, Osaka, Chiba et al.: "Covalent modification of all members of human cullin family proteins by NEDD8." in: Oncogene, Vol. 18, Issue 48, pp. 6829-34, 2000 (PubMed).

Antenos, Casper, Brown: "Interaction with Nedd8, a ubiquitin-like protein, enhances the transcriptional activity of the aryl hydrocarbon receptor." in: The Journal of biological chemistry, Vol. 277, Issue 46, pp. 44028-34, 2002 (PubMed).

Carrabino, Carminati, Talarico et al.: "Expression pattern of the JAB1/CSN5 gene during murine embryogenesis: colocalization with NEDD8." in: Gene expression patterns : GEP, Vol. 4, Issue 4, pp. 423-31, 2004 (PubMed).