NEDD8 antibody (Neural Precursor Cell Expressed, Developmentally Down-Regulated 8) (N-Term)

Details for Product anti-NEDD8 Antibody No. ABIN350513, Supplier: Log in to see
Antigen
  • CG10679
  • Dmel\\CG10679
  • NEDD8
  • dNEDD8
  • nedd8
  • ubiquitin
  • si:zc14a17.5
  • GB17785
  • DDBDRAFT_0218116
  • DDBDRAFT_0238041
  • DDB_0218116
  • DDB_0238041
  • NEDD-8
  • Rub1
  • CG10679 gene product from transcript CG10679-RA
  • neural precursor cell expressed, developmentally down-regulated 8
  • Nedd8
  • ubiquitin-like protein Nedd8
  • NEDD8 protein
  • NEDD8-like
  • neural precursor cell expressed, developmentally down-regulated gene 8
  • Nedd8
  • nedd8
  • NEDD8
  • LOC100520086
Epitope
N-Term
31
5
4
4
3
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
109
54
51
6
5
5
4
3
2
2
2
2
2
1
1
1
Host
Sheep
83
24
4
Clonality
Polyclonal
Conjugate
This NEDD8 antibody is un-conjugated
3
3
3
2
2
2
1
1
1
1
1
1
1
1
1
1
1
Application
Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
94
53
50
24
19
15
13
11
8
4
4
2
2
2
Options
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Immunogen A synthetic peptide from the n-terminal of mouse NEDD8 conjugated to blue carrier protein has been used as the antigen. The antigen is homologous in human, rat, zebrafish, drosophila and xenopus.
Specificity Specific for NEDD8.
Purification Whole serum
Alternative Name NEDD8 (NEDD8 Antibody Abstract)
Background Function: Ubiquitin-like protein which plays an important role in cell cycle control and embryogenesis. Covalent attachment to its substrates requires prior activation by the E1 complex UBE1C-APPBP1 and linkage to the E2 enzyme UBE2M. Attachment of NEDD8 to cullins activates their associated E3 ubiquitin ligase activity, and thus promotes polyubiquitination and proteasomal degradation of cyclins and other regulatory protein.
Subcellular location: Mainly nuclear
Tissue specificity: Ubiquitously expressed (at protein level). Post-translational modification: Cleavage of precursor form by UCHL3 or SENP8 is necessary for function Also known as: Ubiquitin-like protein Nedd8, Neddylin, Neural precursor cell expressed developmentally down-regulated protein 8, NEDD-8.
Research Area Proteolysis / Ubiquitin
Application Notes Use at a dilution of 1 : 300 to 1 : 2000.
The optimal dilution should be determined by the end user.
Not yet tested in other applications.
Restrictions For Research Use only
Format Lyophilized
Reconstitution Reconstitute in 500 µL of sterile water. Centrifuge to remove any insoluble material.
Handling Advice Avoid freeze and thaw cycles.
Storage 4 °C/-20 °C
Storage Comment Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.
Expiry Date 12 months
Background publications Carrabino, Carminati, Talarico, Pardi, Bianchi: "Expression pattern of the JAB1/CSN5 gene during murine embryogenesis: colocalization with NEDD8." in: Gene expression patterns : GEP, Vol. 4, Issue 4, pp. 423-31, 2004 (PubMed).

Antenos, Casper, Brown: "Interaction with Nedd8, a ubiquitin-like protein, enhances the transcriptional activity of the aryl hydrocarbon receptor." in: The Journal of biological chemistry, Vol. 277, Issue 46, pp. 44028-34, 2002 (PubMed).

Hori, Osaka, Chiba, Miyamoto, Okabayashi, Shimbara, Kato, Tanaka: "Covalent modification of all members of human cullin family proteins by NEDD8." in: Oncogene, Vol. 18, Issue 48, pp. 6829-34, 2000 (PubMed).

Kumar, Yoshida, Noda: "Cloning of a cDNA which encodes a novel ubiquitin-like protein." in: Biochemical and biophysical research communications, Vol. 195, Issue 1, pp. 393-9, 1993 (PubMed).

Kumar, Tomooka, Noda: "Identification of a set of genes with developmentally down-regulated expression in the mouse brain." in: Biochemical and biophysical research communications, Vol. 185, Issue 3, pp. 1155-61, 1992 (PubMed).