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NG2 antibody (Melanoma Associated Chondroitin Sulfate Proteoglycan) (Extracellular Domain)

Details for Product anti-MCSP Antibody No. ABIN350515, Supplier: Log in to see
Antigen
  • 2410131O05Rik
  • 4732461B14Rik
  • AA407576
  • AN2
  • HMW-MAA
  • MCSP
  • MCSPG
  • MEL-CSPG
  • MSK16
  • NG2
  • Ng2
Epitope
Extracellular Domain, AA 2080-2130
14
8
7
5
5
5
4
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Mouse (Murine)
77
68
53
2
Host
Rabbit
82
16
2
Clonality
Polyclonal
Conjugate
This NG2 antibody is un-conjugated
9
7
3
3
3
3
3
3
3
3
3
3
2
1
Application
Immunohistochemistry (IHC), Western Blotting (WB)
42
33
29
15
13
10
10
9
8
4
1
Supplier
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Immunogen A synthetic peptide from aa region 2080-2130 of the extracellular domain of mouse NG2 conjugated to blue carrier protein was used as the antigen.
Specificity Specific for NG2.
Purification Whole serum
Alternative Name NG2 (MCSP Antibody Abstract)
Background Valuable marker for several incompletely differentiated precursor cells.
Function: Proteoglycan playing a role in cell proliferation and migration which stimulates endothelial cells motility during microvascular morphogenesis. May also inhibit neurite outgrowth and growth cone collapse during axon regeneration. Cell surface receptor for collagen alpha 2(VI) which may confer cells ability to migrate on that substrate. Binds through its extracellular N-terminus growth factors, extracellular matrix proteases modulating their activity. May regulate MPP16-dependent degradation and invasion of type I collagen participating in melanoma cells invasion properties. May modulate the plasminogen system by enhancing plasminogen activation and inhibiting angiostatin. Functions also as a signal transducing protein by binding through its cytoplasmic C-terminus scaffolding and signaling proteins. May promote retraction fiber formation and cell polarization through Rho GTPase activation. May stimulate alpha-4, beta-1 integrin-mediated adhesion and spreading by recruiting and activating a signaling cascade through CDC42, ACK1 and BCAR1. May activate FAK and ERK1/ERK2 signaling cascades.
Subcellular location: Apical cell membrane, Single-pass type I membrane protein, Extracellular side. Cell projection › lamellipodium membrane, Single-pass type I membrane protein, Extracellular side.
Tissue specificity: Expressed in microcascular pericytes and not endothelial cells. Also known as: MEL CSPG, MELCSPG, Chondroitin sulfate proteoglycan 4, Chondroitin sulfate proteoglycan NG2, CSPG4, MCSP, MCSPG, Melanoma associated chondroitin sulfate proteoglycan, Melanoma chondroitin sulfate proteoglycan, MSK16.
Research Area Cancer, Glia marker
Application Notes A dilution of 1 : 300 to 1 : 2000 is recommended.
The optimal dilution should be determined by the end user.
Not yet tested in other applications.
Restrictions For Research Use only
Format Lyophilized
Reconstitution Reconstitute in 150 µL of sterile water. Centrifuge to remove any insoluble material.
Handling Advice Avoid freeze and thaw cycles.
Storage 4 °C/-20 °C
Storage Comment Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.
Expiry Date 12 months
Background publications Zahedi, Lewandrowski, Wiesner, Wortelkamp, Moebius, Schütz, Walter, Gambaryan, Sickmann: "Phosphoproteome of resting human platelets." in: Journal of proteome research, Vol. 7, Issue 2, pp. 526-34, 2008

Gregory, Barlow, McLay, Kaul, Swarbreck, Dunham, Scott, Howe, Woodfine, Spencer, Jones, Gillson, Searle, Zhou, Kokocinski, McDonald, Evans, Phillips, Atkinson, Cooper, Jones, Hall, Andrews, Lloyd et al.: "The DNA sequence and biological annotation of human chromosome 1. ..." in: Nature, Vol. 441, Issue 7091, pp. 315-21, 2006

Teng, Teng, Lee, Wright, Tevar, Almeida, Kermani, Torkin, Chen, Lee, Kraemer, Nykjaer, Hempstead: "ProBDNF induces neuronal apoptosis via activation of a receptor complex of p75NTR and sortilin." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 25, Issue 22, pp. 5455-63, 2005

Martin, Vincent, Mazella: "Involvement of the neurotensin receptor-3 in the neurotensin-induced migration of human microglia." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 23, Issue 4, pp. 1198-205, 2003

Maeda, Nobukuni, Shimo-Onoda, Hayashi, Yone, Komiya, Inoue: "Sortilin is upregulated during osteoblastic differentiation of mesenchymal stem cells and promotes extracellular matrix mineralization." in: Journal of cellular physiology, Vol. 193, Issue 1, pp. 73-9, 2002

Shiba, Takatsu, Nogi, Matsugaki, Kawasaki, Igarashi, Suzuki, Kato, Earnest, Nakayama, Wakatsuki: "Structural basis for recognition of acidic-cluster dileucine sequence by GGA1." in: Nature, Vol. 415, Issue 6874, pp. 937-41, 2002

Nielsen, Madsen, Christensen, Nykjaer, Gliemann, Kasper, Pohlmann, Petersen: "The sortilin cytoplasmic tail conveys Golgi-endosome transport and binds the VHS domain of the GGA2 sorting protein." in: The EMBO journal, Vol. 20, Issue 9, pp. 2180-90, 2001

Nielsen, Jacobsen, Olivecrona, Gliemann, Petersen: "Sortilin/neurotensin receptor-3 binds and mediates degradation of lipoprotein lipase." in: The Journal of biological chemistry, Vol. 274, Issue 13, pp. 8832-6, 1999

Mazella, Zsürger, Navarro, Chabry, Kaghad, Caput, Ferrara, Vita, Gully, Maffrand, Vincent: "The 100-kDa neurotensin receptor is gp95/sortilin, a non-G-protein-coupled receptor." in: The Journal of biological chemistry, Vol. 273, Issue 41, pp. 26273-6, 1998

Petersen, Nielsen, Nykjaer, Jacobsen, Tommerup, Rasmussen, Roigaard, Gliemann, Madsen, Moestrup: "Molecular identification of a novel candidate sorting receptor purified from human brain by receptor-associated protein affinity chromatography." in: The Journal of biological chemistry, Vol. 272, Issue 6, pp. 3599-605, 1997