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Glutamate Receptor, Ionotropic, N-Methyl D-Aspartate 2a (GRIN2A) (2nd Cytoplasmic Domain) antibody

Antigen

Glutamate Receptor, Ionotropic, N-Methyl D-Aspartate 2a (GRIN2A)
Synonyms GRIN2A, NMDAR2A, NR2A, nr2a
Binding Site
Alternatives

2nd Cytoplasmic Domain
Clonality Polyclonal
Host
Alternatives

Rabbit
Reactivity
Conjugate
Alternatives Un-conjugated
Application
Alternatives Immunohistochemistry (IHC), Western Blotting (WB)
5 references available
Catalog no. ABIN350517
Quantity 100 µl  (Variants)
Price 454.67 $   Plus shipping costs $45.00
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Additional Information
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Alternative name NMDAR2A (GRIN2A)
UniProt Q12879
Immunogen A synthetic peptide from the 2d cytoplasmic domain of human NMDAR2A (GRIN2A) conjugated to an immunogenic carrier protein was used as the immunogen.
Format Lyophilized
Description FUNCTION: NMDA receptor subtype of glutamate-gated ion channels possesses high calcium permeability and voltage-dependent sensitivity to magnesium. Activation requires binding of agonist to both types of subunits. Subcellular location: Cell membrane; Multi-pass membrane protein. Cell junction › synapse › postsynaptic cell membrane; Multi-pass membrane protein. Also known as: GRIN2A, Glutamate receptor subunit epsilon-1, Glutamate [NMDA] receptor subunit epsilon-1, N-methyl D-aspartate receptor subtype 2A, NMDE1, NR2A.
Specificity Appears to be specific for NMDAR2A.

Application Details
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Application Notes IHC, WB. A dilution of 1 : 300 to 1 : 2000 is recommended. The optimal dilution should be determined by the end user. Not yet tested in other applications.
Purity whole serum
Purification The antibody is affinity-purified from rabbit antiserum by affinity chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide is removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Storage Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.
Restrictions For Research Use only

Publications
Application Details Alternatives Variants back to top

Foldes, Adams, Fantaske et al.: "Human N-methyl-D-aspartate receptor modulatory subunit hNR2A: cloning and sequencing of the cDNA and primary structure of the protein." in: Biochimica et biophysica acta, Vol. 1223, Issue 1, pp. 155-9, 1994 (PubMed).

Hess, Daggett, Crona et al.: "Cloning and functional characterization of human heteromeric N-methyl-D-aspartate receptors." in: The Journal of pharmacology and experimental therapeutics, Vol. 278, Issue 2, pp. 808-16, 1996 (PubMed).

Olsen, Blagoev, Gnad et al.: "Global, in vivo, and site-specific phosphorylation dynamics in signaling networks." in: Cell, Vol. 127, Issue 3, pp. 635-48, 2006 (PubMed).

Munton, Tweedie-Cullen, Livingstone-Zatchej et al.: "Qualitative and quantitative analyses of protein phosphorylation in naive and stimulated mouse synaptosomal preparations." in: Molecular & cellular proteomics : MCP, Vol. 6, Issue 2, pp. 283-93, 2007 (PubMed).

Molina, Horn, Tang et al.: "Global proteomic profiling of phosphopeptides using electron transfer dissociation tandem mass spectrometry." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 104, Issue 7, pp. 2199-204, 2007 (PubMed).

Alternatives
Application Details Publications Variants back to top

Alternatives for antigen "Glutamate Receptor, Ionotropic, N-Methyl D-Aspartate 2a (GRIN2A)", type "Antibodies"
Hosts Rabbit (51), Mouse (5), Goat (1)
Reactivities Human (44), Rat (Rattus) (37), Mouse (Murine) (35), Cat (Feline) (1), Chicken (1), Cow (Bovine) (1), Dog (Canine) (1)
Applications Western Blotting (WB) (31), Immunofluorescence (IF) (28), ELISA (9), Immunohistochemistry (IHC) (8), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (7), Immunoprecipitation (IP) (5), Immunocytochemistry (ICC) (3), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (3), Immunostaining (ISt) (3), Immunohistochemistry (Fixed) (IHC (fx)) (2), Immunoelectron Microscopy (IEM) (1)
Conjugates Alexa Fluor 350 (3), Alexa Fluor 488 (3), Alexa Fluor 555 (3), Alexa Fluor 647 (3), PE,Cy5.5 (3), Biotin (1), Cy3 (1), Cy5 (1), Cy5.5 (1), Cy7 (1), FITC (1), Gold (1), HRP (1), PE (1), PE,Cy3 (1), PE,Cy5 (1), PE,Cy7 (1)
Epitopes C-Term (8), pTyr1246 (5), pTyr1325 (4), 2nd Cytoplasmic Domain (1), AA 1099-1213 (1), AA 1265-1464 (1), AA 875-885 (1), Center (1), Cytoplasmic Domain (1), Internal Region (1), N-Term (1), pTyr771 (1)