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Nitric Oxide Synthase 1, Neuronal (NOS1) (C-Term) antibody

Details for Product No. ABIN350523, Supplier: Log in to see
Antigen
  • nos
  • nNOS
  • NOS1
  • IHPS1
  • N-NOS
  • NC-NOS
  • NOS
  • bNOS
  • 2310005C01Rik
  • NO
  • NOS-I
  • Nos-1
Epitope
C-Term
44
15
14
13
8
7
6
6
6
6
6
6
6
6
5
5
5
5
4
3
3
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Rat (Rattus), Mouse (Murine), Human, Rabbit, Guinea Pig
172
107
77
8
7
6
5
5
5
2
2
1
1
1
1
Host
Goat
192
19
18
3
2
1
Clonality
Polyclonal
Conjugate
Un-conjugated
13
11
4
3
3
3
2
2
2
1
1
1
1
1
1
1
1
Application
Immunohistochemistry (IHC), Western Blotting (WB)
154
93
52
41
31
24
23
21
19
12
7
2
1
1
Supplier
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Immunogen A synthetic pentamer-peptide from the c-terminal region of human NOS1 conjugated to an immunogenic carrier protein was used as the antigen.
Specificity Specific for NOS1.
Purification Whole serum
Alternative Name NOS1 (NOS1 Antibody Abstract)
Background Function: Produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body. In the brain and peripheral nervous system, NO displays many properties of a neurotransmitter. Inhibitory transmitter for non-adrenergic and non-cholinergic nerves in the colorectum.
Catalytic activity: L-arginine + n NADPH + m O2 = citrulline + nitric oxide + n NADP+.
Enzyme regulation: Stimulated by calcium/calmodulin. Inhibited by n-Nos-inhibiting protein (PIN) which may prevent the dimerization of the protein. Inhibited by NOSIP.
Subcellular location: Cell membrane, sarcolemma, Peripheral membrane protein. Cell projection, dendritic spine. Note=In skeletal muscle, it is localized beneath the sarcolemma of fast-twitch muscle fiber by associating with the dystrophin glycoprotein complex. In neurons, enriched in dendritic spines.
Tissue specificity: Isoform N-NOS-1 is expressed in brain and colorectum. Found in the Auerbach's plexus of the enteric nervous system. Isoform PNNOS is expressed in the penis, urethra, prostate, and skeletal muscle, and coexists with the cerebellar nnos in the pelvic plexus, bladder and liver, and is detectable in the cerebellum. Also known as: BNOS, NOS type I, Neuronal NOS, N-NOS, nNOS, Constitutive NOS, NC-NOS, Nitric oxide synthase.
Research Area Neurotransmitters
Pathways
Application Notes A dilution of 1: 300 to 1: 3000 is recommended.
The optimal dilution should be determined by the end user.
Restrictions For Research Use only
Format Lyophilized
Reconstitution Reconstitute in 100 µL of sterile water. Centrifuge to remove any insoluble material.
Handling Advice Avoid freeze and thaw cycles.
Storage 4 °C/-20 °C
Storage Comment Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.
Expiry Date 12 months
Background publications Jaffrey, Benfenati, Snowman et al.: "Neuronal nitric-oxide synthase localization mediated by a ternary complex with synapsin and CAPON." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 99, Issue 5, pp. 3199-204, 2002 (PubMed).

Sato, Sagami, Matsui et al.: "Unusual role of Tyr588 of neuronal nitric oxide synthase in controlling substrate specificity and electron transfer." in: Biochemical and biophysical research communications, Vol. 281, Issue 3, pp. 621-6, 2001 (PubMed).

Fang, Jaffrey, Sawa et al.: "Dexras1: a G protein specifically coupled to neuronal nitric oxide synthase via CAPON." in: Neuron, Vol. 28, Issue 1, pp. 183-93, 2000 (PubMed).

Magee, Fuentes, Garban et al.: "Cloning of a novel neuronal nitric oxide synthase expressed in penis and lower urinary tract." in: Biochemical and biophysical research communications, Vol. 226, Issue 1, pp. 145-51, 1996 (PubMed).

Uvarov VYu, Lyashenko: "The identification of the pterin-binding domain in the nitric oxide synthase's sequence." in: Biochemical and biophysical research communications, Vol. 206, Issue 2, pp. 736-41, 1995 (PubMed).

Seo, Tatsumi, Fujii et al.: "Nitric oxide synthase from rat colorectum: purification, peptide sequencing, partial PCR cloning, and immunohistochemistry." in: Journal of biochemistry, Vol. 115, Issue 3, pp. 602-7, 1994 (PubMed).