NOS1 (Neuronal NOS, Constitutive NOS) (C-Term) antibody

Antigen: NOS1 (Neuronal NOS, Constitutive NOS)

Binding Site: C-Term

Clonality: Polyclonal

Host: Goat

Application: Immunohistochemistry (IHC), Western Blotting (WB)

Catalog no.: ABIN350524

Additional Information

UniProt: P29476

Immunogen: A synthetic pentamer-peptide from the c-terminal part of rat, mouse, human, rabbit, guinea pig NOS1 (Nitric oxide synthase, BNOS, Neuronal NOS, Constitutive NOS) conjugated to an immunogenic carrier protein was used as the immunogen.

Format: Lyophilized

Isotype: IgG

Description: FUNCTION: Produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body. In the brain and peripheral nervous system, NO displays many properties of a neurotransmitter. Inhibitory transmitter for non-adrenergic and non-cholinergic nerves in the colorectum. CATALYTIC ACTIVITY: L-arginine + n NADPH + m O2 = citrulline + nitric oxide + n NADP+. ENZYME REGULATION: Stimulated by calcium/calmodulin. Inhibited by n-Nos-inhibiting protein (PIN) which may prevent the dimerization of the protein. Inhibited by NOSIP. SUBCELLULAR LOCATION: Cell membrane, sarcolemma; Peripheral membrane protein. Cell projection, dendritic spine. Note=In skeletal muscle, it is localized beneath the sarcolemma of fast-twitch muscle fiber by associating with the dystrophin glycoprotein complex. In neurons, enriched in dendritic spines. TISSUE SPECIFICITY: Isoform N-NOS-1 is expressed in brain and colorectum. Found in the Auerbach's plexus of the enteric nervous system. Isoform PNNOS is expressed in the penis, urethra, prostate, and skeletal muscle, and coexists with the cerebellar nnos in the pelvic plexus, bladder and liver, and is detectable in the cerebellum. Also known as: BNOS, NOS type I, Neuronal NOS, N-NOS, nNOS, Constitutive NOS, NC-NOS, Nitric oxide synthase.

Specificity: Appears to be specific for NOS1.

Application Details

Application Notes: IHC, WB. A concentration of 10-50 µg/ml is recommended. The optimal concentration should be determined by the end user.

Purity: IgG

Purification: The antibody is affinity-purified from rabbit antiserum by affinity chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide is removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

Storage: Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.

Restrictions: For Research Use only

Publications

Seo, Tatsumi, Fujii et al.: "Nitric oxide synthase from rat colorectum: purification, peptide sequencing, partial PCR cloning, and immunohistochemistry." in: Journal of biochemistry, Vol. 115, Issue 3, pp. 602-7, 1994 (PubMed).

Uvarov VYu, Lyashenko: "The identification of the pterin-binding domain in the nitric oxide synthase's sequence." in: Biochemical and biophysical research communications, Vol. 206, Issue 2, pp. 736-41, 1995 (PubMed).

Magee, Fuentes, Garban et al.: "Cloning of a novel neuronal nitric oxide synthase expressed in penis and lower urinary tract." in: Biochemical and biophysical research communications, Vol. 226, Issue 1, pp. 145-51, 1996 (PubMed).

Fang, Jaffrey, Sawa et al.: "Dexras1: a G protein specifically coupled to neuronal nitric oxide synthase via CAPON." in: Neuron, Vol. 28, Issue 1, pp. 183-93, 2000 (PubMed).

Sato, Sagami, Matsui et al.: "Unusual role of Tyr588 of neuronal nitric oxide synthase in controlling substrate specificity and electron transfer." in: Biochemical and biophysical research communications, Vol. 281, Issue 3, pp. 621-6, 2001 (PubMed).

Jaffrey, Benfenati, Snowman et al.: "Neuronal nitric-oxide synthase localization mediated by a ternary complex with synapsin and CAPON." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 99, Issue 5, pp. 3199-204, 2002 (PubMed).